ArticleGastroenterology2007
Allergen-IgE complexes trigger CD23-dependent CCL20 release from human intestinal epithelial cells.
Article in Gastroenterology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 49 citations in OpenAlex.
- A Comparison of Natural and Therapeutic Anti-IgE Antibodies.Antibodies (Basel, Switzerland) · 2024Review
- Prenatal Factors in the Development of Allergic Diseases.International journal of molecular sciences · 2024Review
- Is Wheat Glutenin Extract Intrinsically Allergenic? Evaluation Using a Novel Adjuvant-Free Mouse Model of Systemic Anaphylaxis.International journal of molecular sciences · 2023Article
- Chronic application of alcohol-soluble gluten extract over undamaged skin causes clinical sensitization for life-threatening anaphylaxis via activation of systemic Th2 immune responses in mice.Frontiers in allergy · 2023Article
- Bidirectional Transport of IgE by CD23 in the Inner Ear of Patients with Meniere's Disease.Journal of immunology (Baltimore, Md. : 1950) · 2022Article
- Highly-metastatic colorectal cancer cell released miR-181a-5p-rich extracellular vesicles promote liver metastasis by activating hepatic stellate cells and remodelling the tumour microenvironment.Journal of extracellular vesicles · 2022Article
- Mechanisms of Immunotoxicity: Stressors and Evaluators.International journal of molecular sciences · 2021Review
- Review
- Role of the Intestinal Epithelium and Its Interaction With the Microbiota in Food Allergy.Frontiers in immunology · 2020Review
- Gemcitabine enhances rituximab-mediated complement-dependent cytotoxicity to B cell lymphoma by CD20 upregulation.Cancer science · 2016Article
- Inhibition of CD23-mediated IgE transcytosis suppresses the initiation and development of allergic airway inflammation.Mucosal immunology · 2015Article
- Article
- Serum levels of the CC chemokines CCL2, CCL5, and CCL11 in food allergic children with different clinical manifestations.Inflammation · 2013Article
- In vivo intranasal anti-CD23 treatment inhibits allergic responses in a murine model of allergic rhinitis.Journal of molecular histology · 2013Article
- Mucosal immunology of tolerance and allergy in the gastrointestinal tract.Immunologic research · 2012Review
- Mucosal antibodies in the regulation of tolerance and allergy to foods.Seminars in immunopathology · 2012Review
- Article
- Is there a regulatory role of immunoglobulins on tissue forming cells relevant in chronic inflammatory lung diseases?Journal of allergy · 2011Article
- Quercetin and kaempferol suppress immunoglobulin E-mediated allergic inflammation in RBL-2H3 and Caco-2 cells.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2010Article
- A functional role for CCR6 on proallergic T cells in the gastrointestinal tract.Gastroenterology · 2010Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
BACKGROUND &
aimsIn food allergic individuals, exposure to food allergens by the oral route can trigger immediate (within minutes) local hypersensitivity reactions in the intestine followed by a late-phase inflammatory response. Previous work has shown that CD23 is constitutively expressed by human intestinal epithelial cells and mediates the uptake of allergen-IgE complexes. We hypothesized that allergen-IgE complexes could also signal via CD23 to trigger an inflammatory cascade in the local environment.
methodsCaco-2 monolayers were stimulated with human IgE-antigen (Ag) complexes. IL-8 and CCL20 mRNA and protein were determined by RT-PCR and ELISA, respectively. Signaling pathways were assessed by immunoblotting. Endogenous CD23 expression was knocked down by stable transfection with CD23 shRNA retroviral plasmid. Migration assays were performed using human monocyte-derived dendritic cells.
resultsStimulation of Caco-2 cells with IgE-Ag complexes triggered upregulation of IL-8 and CCL20 at the mRNA and protein level. Allergen complexes induced phosphorylation of ERK and JNK, but not p38 MAP kinase or NK-kappaB, and resulted in AP-1 activation. Cross-linking of CD23 replicated the findings with IgE-Ag complexes, and silencing of CD23 expression abrogated the response to allergen-IgE complexes. Supernatant from IgE-Ag-stimulated epithelial cells induced migration of dendritic cells in a CCL20-dependent manner. Finally, immunostaining of duodenal biopsies demonstrated that CCL20 was constitutively expressed by epithelial cells in vivo.
conclusionsSignaling via epithelial CD23 may participate in the late-phase inflammatory response by the release of chemokines capable of recruiting antigen presenting cells and effector cells of allergic inflammation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.