Evidence map›Paper›PMID 1805207›Full record

ArticleParasitology research1991

Giardia duodenalis: a freeze-fracture, fracture-flip and cytochemistry study.

W M Kattenbach, P F Pimenta, W de Souza, P Pinto da Silva

Abstract read
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In one paragraph

Article in Parasitology research, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed.

  1. Article
  2. Endocytosis in anaerobic parasitic protists.Memorias do Instituto Oswaldo Cruz · 2024
    Review
  3. Parasitology · 2021
    Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Biology of Giardia lamblia.Clinical microbiology reviews · 2001
    Review
  9. Mechanisms of Giardia lamblia differentiation into cysts.Microbiology and molecular biology reviews : MMBR · 1997
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

W M KattenbachDepartamento de Parasitologia e Biofísica Celular, Instituto de Biofísica Carlos Chagas Filho, Brazil.
P F Pimenta
W de Souza
P Pinto da Silva

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The freeze-fracture technique was used to study the structural organization of the membranes of trophozoites of the protozoon Giardia duodenalis. No special array of intramembranous particles was observed in the membrane lining the protozoon body or the flagella. A large globular protuberance located in the ventral region displayed several small circular indentations similar to those seen in the dorsal region. These also occurred on the parasite surface as revealed in fracture-flip replicas. A large number of vesicles were observed below the plasma membrane; they corresponded to an acidic compartment as indicated by fluorescence microscopy of acridine orange-stained cells and contained acid phosphatase as indicated by cytochemistry. In addition, gold-labeled macromolecules (albumin, peroxidase, transferrin, and low-density lipoprotein) accumulated in the vesicles. These observations suggest that the peripheral vesicles of trophozoites are part of the endosomal-lysosomal system of G. duodenalis.

Indexed as

Acid PhosphataseAnimalsCell MembraneFreeze FracturingGiardiaHistocytochemistryMicroscopy, ElectronMicroscopy, Electron, ScanningMicroscopy, FluorescenceAcid Phosphatase

Identifiers

PMID1805207

What OpenQuestion holds

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Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.