ArticleNucleic acids research2008
Mechanisms of covalent self-assembly of the Azoarcus ribozyme from four fragment oligonucleotides.
Article in Nucleic acids research, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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22 citing papers in PubMed, 49 citations in OpenAlex.
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- RNA diversification by a self-reproducing ribozyme revealed by deep sequencing and kinetic modelling.Chemical communications (Cambridge, England) · 2021Article
- Two Sides of the Same Coin: Emergence of Foldamers and Self-Replicators from Dynamic Combinatorial Libraries.Journal of the American Chemical Society · 2021Article
- Self-Reproduction and Darwinian Evolution in Autocatalytic Chemical Reaction Systems.Life (Basel, Switzerland) · 2021Review
- Darwinian properties and their trade-offs in autocatalytic RNA reaction networks.Nature communications · 2021Article
- From self-replication to replicator systems en route to de novo life.Nature reviews. Chemistry · 2020Review
- Coupled catabolism and anabolism in autocatalytic RNA sets.Nucleic acids research · 2018Article
- Corroboration of Zn(ii)-Mg(ii)-tertiary structure interplays essential for the optimal catalysis of a phosphorothiolate thiolesterase ribozyme.RSC advances · 2018Article
- Prebiotic RNA Network Formation: A Taxonomy of Molecular Cooperation.Life (Basel, Switzerland) · 2017Article
- Topological and thermodynamic factors that influence the evolution of small networks of catalytic RNA species.RNA (New York, N.Y.) · 2017Article
- Group I Intron Internal Guide Sequence Binding Strength as a Component of Ribozyme Network Formation.Molecules (Basel, Switzerland) · 2016Article
- Dynamics of prebiotic RNA reproduction illuminated by chemical game theory.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Parasites Sustain and Enhance RNA-Like Replicators through Spatial Self-Organisation.PLoS computational biology · 2016Article
- Speciation of a group I intron into a lariat capping ribozyme.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Article
- Self-replication reactions dependent on tertiary interaction motifs in an RNA ligase ribozyme.Journal of molecular evolution · 2011Article
- Closing the circle: replicating RNA with RNA.Cold Spring Harbor perspectives in biology · 2010Review
- One RNA plays three roles to provide catalytic activity to a group I intron lacking an endogenous internal guide sequence.Nucleic acids research · 2009Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RNA oligomers of length 40-60 nt can self-assemble into covalent versions of the Azoarcus group I intron ribozyme. This process requires a series of recombination reactions in which the internal guide sequence of a nascent catalytic complex makes specific interactions with a complement triplet, CAU, in the oligomers. However, if the CAU were mutated, promiscuous self-assembly may be possible, lessening the dependence on a particular set of oligomer sequences. Here, we assayed whether oligomers containing mutations in the CAU triplet could still self-construct Azoarcus ribozymes. The mutations CAC, CAG, CUU and GAU all inhibited self-assembly to some degree, but did not block it completely in 100 mM MgCl(2). Oligomers containing the CAC mutation retained the most self-assembly activity, while those containing GAU retained the least, indicating that mutations more 5' in this triplet are the most deleterious. Self-assembly systems containing additional mutant locations were progressively less functional. Analyses of properly self-assembled ribozymes revealed that, of two recombination mechanisms possible for self-assembly, termed 'tF2' and 'R2F2', the simpler one-step 'tF2' mechanism is utilized when mutations exist. These data suggest that self-assembling systems are more facile than previously believed, and have relevance to the origin of complex ribozymes during the RNA World.
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