ArticleNucleic acids research2008
The RNA-dependent RNA polymerase essential for post-transcriptional gene silencing in Neurospora crassa interacts with replication protein A.
Article in Nucleic acids research, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 35 citations in OpenAlex.
- Fungal small RNA: unveiling the breakthroughs and promising applications.Frontiers in fungal biology · 2025Review
- Remodeling of perturbed chromatin can initiate de novo transcriptional and post-transcriptional silencing.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Functions of RNAi Pathways in Ribosomal RNA Regulation.Non-coding RNA · 2024Review
- Mycovirus-encoded suppressors of RNA silencing: Possible allies or enemies in the use of RNAi to control fungal disease in crops.Frontiers in fungal biology · 2022Review
- RNA-induced initiation of transcriptional silencing (RITS) complex structure and function.RNA biology · 2019Review
- Mechanism of siRNA production from repetitive DNA.Genes & development · 2015Article
- Histone H3K56 acetylation is required for quelling-induced small RNA production through its role in homologous recombination.The Journal of biological chemistry · 2014Article
- Recognizing the enemy within: licensing RNA-guided genome defense.Trends in biochemical sciences · 2014Review
- Article
- Homologous recombination as a mechanism to recognize repetitive DNA sequences in an RNAi pathway.Genes & development · 2013Article
- Host-induced gene silencing: a tool for understanding fungal host interaction and for developing novel disease control strategies.Molecular plant pathology · 2012Article
- Transgene induced co-suppression during vegetative growth in Cryptococcus neoformans.PLoS genetics · 2012Article
- RNA interference pathways in fungi: mechanisms and functions.Annual review of microbiology · 2012Review
- RNA interference in fungi: pathways, functions, and applications.Eukaryotic cell · 2011Review
- Characterizing the role of RNA silencing components in Cryptococcus neoformans.Fungal genetics and biology : FG & B · 2010Article
- RNA interference pathways in filamentous fungi.Cellular and molecular life sciences : CMLS · 2010Review
- The DNA/RNA-dependent RNA polymerase QDE-1 generates aberrant RNA and dsRNA for RNAi in a process requiring replication protein A and a DNA helicase.PLoS biology · 2010Article
- In vitro activities of the multifunctional RNA silencing polymerase QDE-1 of Neurospora crassa.The Journal of biological chemistry · 2010Article
- QIP, a protein that converts duplex siRNA into single strands, is required for meiotic silencing by unpaired DNA.Genetics · 2010Article
- Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Post-transcriptional gene silencing (PTGS) pathways play a role in genome defence and have been extensively studied, yet how repetitive elements in the genome are identified is still unclear. It has been suggested that they may produce aberrant transcripts (aRNA) that are converted by an RNA-dependent RNA polymerase (RdRP) into double-stranded RNA (dsRNA), the essential intermediate of PTGS. However, how RdRP enzymes recognize aberrant transcripts remains a key question. Here we show that in Neurospora crassa the RdRP QDE-1 interacts with Replication Protein A (RPA), part of the DNA replication machinery. We show that both QDE-1 and RPA are nuclear proteins and that QDE-1 is specifically recruited onto the repetitive transgenic loci. We speculate that this localization of QDE-1 could allow the in situ production of dsRNA using transgenic nascent transcripts as templates, as in other systems. Supporting a link between the two proteins, we found that the accumulation of short interfering RNAs (siRNAs), the hallmark of silencing, is dependent on an ongoing DNA synthesis. The interaction between QDE-1 and RPA is important since it should guide further studies aimed at understanding the specificity of the RdRP and it provides for the first time a potential link between a PTGS component and the DNA replication machinery.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.