ArticleBMC bioinformatics2007
Gene expression changes induced by the tumorigenic pyrrolizidine alkaloid riddelliine in liver of Big Blue rats.
Article in BMC bioinformatics, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 56 citations in OpenAlex.
- A Biomarker-Driven Ovary-Endometrium Organ-on-a-Chip Mimicking 3D Multicellular Complexity and Menstrual Cyclicity for Predicting Reproductive Toxicity.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Advanced Lung-on-a-Chip Technology: Mimicking the Complex Human Lung Microenvironment.International journal of biological sciences · 2025Article
- Development of a novel testis-on-a-chip that demonstrates reciprocal crosstalk between Sertoli and Leydig cells in testicular tissue.Experimental & molecular medicine · 2024Article
- Development of Advanced Oral-on-a-Chip: Replicating the Intricate Human Oral Microenvironment.International journal of biological sciences · 2024Article
- Pyrrolizidine alkaloids cause cell cycle and DNA damage repair defects as analyzed by transcriptomics in cytochrome P450 3A4-overexpressing HepG2 clone 9 cells.Cell biology and toxicology · 2022Article
- Evaluation of pyrrolizidine alkaloid-induced genotoxicity using metabolically competent TK6 cell lines.Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association · 2020Article
- SERPINB2 Is a Novel Indicator of Cancer Stem Cell Tumorigenicity in Multiple Cancer Types.Cancers · 2019Article
- SERPINB2 is a novel indicator of stem cell toxicity.Cell death & disease · 2018Article
- Genomic analysis of Ugandan and Rwandan chicken ecotypes using a 600 k genotyping array.BMC genomics · 2016Article
- Toxic phytochemicals and their potential risks for human cancer.Cancer prevention research (Philadelphia, Pa.) · 2015Review
- Genotoxicity of pyrrolizidine alkaloids.Journal of applied toxicology : JAT · 2010Review
- Gene expression profiling as an initial approach for mechanistic studies of toxicity and tumorigenicity of herbal plants and herbal dietary supplements.Journal of environmental science and health. Part C, Environmental carcinogenesis & ecotoxicology reviews · 2010Review
- Toxicogenomic biomarkers for liver toxicity.Journal of toxicologic pathology · 2009Article
- Analysis of gene expression changes of drug metabolizing enzymes in the livers of F344 rats following oral treatment with kava extract.Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association · 2009Article
- Proceedings of the 2008 MidSouth Computational Biology and Bioinformatics Society (MCBIOS) Conference.BMC bioinformatics · 2008Article
- Comparison of gene expression profiles altered by comfrey and riddelliine in rat liver.BMC bioinformatics · 2007Article
- Proceedings of the Fourth Annual Conference of the MidSouth Computational Biology and Bioinformatics Society.BMC bioinformatics · 2007Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPyrrolizidine alkaloids (PAs) are probably the most common plant constituents that poison livestock, wildlife, and humans worldwide. Riddelliine is isolated from plants grown in the western United States and is a prototype of genotoxic PAs. Riddelliine was used to investigate the genotoxic effects of PAs via analysis of gene expression in the target tissue of rats in this study. Previously we observed that the mutant frequency in the liver of rats gavaged with riddelliine was 3-fold higher than that in the control group. Molecular analysis of the mutants indicated that there was a statistically significant difference between the mutational spectra from riddelliine-treated and control rats.
resultsRiddelliine-induced gene expression profiles in livers of Big Blue transgenic rats were determined. The female rats were gavaged with riddelliine at a dose of 1 mg/kg body weight 5 days a week for 12 weeks. Rat whole genome microarray was used to perform genome-wide gene expression studies. When a cutoff value of a two-fold change and a P-value less than 0.01 were used as gene selection criteria, 919 genes were identified as differentially expressed in riddelliine-treated rats compared to the control animals. By analysis with the Ingenuity Pathway Analysis Network, we found that these significantly changed genes were mainly involved in cancer, cell death, tissue development, cellular movement, tissue morphology, cell-to-cell signaling and interaction, and cellular growth and proliferation. We further analyzed the genes involved in metabolism, injury of endothelial cells, liver abnormalities, and cancer development in detail.
conclusionThe alterations in gene expression were directly related to the pathological outcomes reported previously. These results provided further insight into the mechanisms involved in toxicity and carcinogenesis after exposure to riddelliine, and permitted us to investigate the interaction of gene products inside the signaling networks.
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