ReviewGenome biology2007
Technology transfer from worms and flies to vertebrates: transposition-based genome manipulations and their future perspectives.
Review in Genome biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
31 citing papers in PubMed.
- Random Insertion Reporter Gimmicks Powered by Cut-and-Paste DNA Transposons.Biomedicines · 2025Review
- Progress of Transposon Vector System for Production of Recombinant Therapeutic Proteins in Mammalian Cells.Frontiers in bioengineering and biotechnology · 2022Review
- Transposon vector-mediated stable gene transfer for the accelerated establishment of recombinant mammalian cell pools allowing for high-yield production of biologics.Biotechnology letters · 2020Review
- Nanotechnology Promotes Genetic and Functional Modifications of Therapeutic T Cells Against Cancer.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2020Review
- Transposons As Tools for Functional Genomics in Vertebrate Models.Trends in genetics : TIG · 2017Review
- Genome-wide analysis of transposable elements in the coffee berry borer Hypothenemus hampei (Coleoptera: Curculionidae): description of novel families.Molecular genetics and genomics : MGG · 2017Article
- Assessing Tn5 and Sleeping Beauty for transpositional transgenesis by cytoplasmic injection into bovine and ovine zygotes.PloS one · 2017Article
- Utilization of Site-Specific Recombination in Biopharmaceutical Production.Iranian biomedical journal · 2016Review
- Genome ARTIST: a robust, high-accuracy aligner tool for mapping transposon insertions and self-insertions.Mobile DNA · 2016Article
- Exogenous enzymes upgrade transgenesis and genetic engineering of farm animals.Cellular and molecular life sciences : CMLS · 2015Review
- Article
- Transposon activation mutagenesis as a screening tool for identifying resistance to cancer therapeutics.BMC cancer · 2013Article
- Genomic deletion induced by Tol2 transposon excision in zebrafish.Nucleic acids research · 2013Article
- Genetic manipulation of schistosomes--progress with integration competent vectors.Parasitology · 2012Review
- Sleeping Beauty transposon mutagenesis of the rat genome in spermatogonial stem cells.Methods (San Diego, Calif.) · 2011Article
- Reliable transgene-independent method for determining Sleeping Beauty transposon copy numbers.Mobile DNA · 2011Article
- Horizontal gene transfers with or without cell fusions in all categories of the living matter.Advances in experimental medicine and biology · 2011Review
- Germline transgenic pigs by Sleeping Beauty transposition in porcine zygotes and targeted integration in the pig genome.PloS one · 2011Article
- Article
- Generating knockout rats by transposon mutagenesis in spermatogonial stem cells.Nature methods · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To meet the increasing demand of linking sequence information to gene function in vertebrate models, genetic modifications must be introduced and their effects analyzed in an easy, controlled, and scalable manner. In the mouse, only about 10% (estimate) of all genes have been knocked out, despite continuous methodologic improvement and extensive effort. Moreover, a large proportion of inactivated genes exhibit no obvious phenotypic alterations. Thus, in order to facilitate analysis of gene function, new genetic tools and strategies are currently under development in these model organisms. Loss of function and gain of function mutagenesis screens based on transposable elements have numerous advantages because they can be applied in vivo and are therefore phenotype driven, and molecular analysis of the mutations is straightforward. At present, laboratory harnessing of transposable elements is more extensive in invertebrate models, mostly because of their earlier discovery in these organisms. Transposons have already been found to facilitate functional genetics research greatly in lower metazoan models, and have been applied most comprehensively in Drosophila. However, transposon based genetic strategies were recently established in vertebrates, and current progress in this field indicates that transposable elements will indeed serve as indispensable tools in the genetic toolkit for vertebrate models. In this review we provide an overview of transposon based genetic modification techniques used in higher and lower metazoan model organisms, and we highlight some of the important general considerations concerning genetic applications of transposon systems.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.