ArticleBMC microbiology2007
Functional, genetic and bioinformatic characterization of a calcium/calmodulin kinase gene in Sporothrix schenckii.
Article in BMC microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 33 citations in OpenAlex.
- Genetic Manipulation inJournal of fungi (Basel, Switzerland) · 2026Review
- Current Insights intoJournal of fungi (Basel, Switzerland) · 2025Article
- Extracutaneous sporotrichosis.Clinical microbiology reviews · 2025Review
- Current Progress inJournal of fungi (Basel, Switzerland) · 2023Review
- Interaction of calcium responsive proteins and transcriptional factors with the PHO regulon in yeasts and fungi.Frontiers in cell and developmental biology · 2023Review
- Characterization and Functional Analysis of a New Calcium/Calmodulin-Dependent Protein Kinase (CaMK1) in the Citrus Pathogenic FungusJournal of fungi (Basel, Switzerland) · 2022Article
- Virulence Factors ofJournal of fungi (Basel, Switzerland) · 2022Review
- Transcriptome Analysis of Dimorphic Fungus Sporothrix schenckii Exposed to Temperature Stress.International microbiology : the official journal of the Spanish Society for Microbiology · 2021Article
- Improvement of the Biosynthesis of Resveratrol in Endophytic Fungus (Frontiers in microbiology · 2021Article
- Identification of a novel fused gene family implicates convergent evolution in eukaryotic calcium signaling.BMC genomics · 2018Article
- Article
- Construction and analysis of the cDNA subtraction library of yeast and mycelial phases of Sporothrix globosa isolated in China: identification of differentially expressed genes.Journal of Zhejiang University. Science. B · 2015Article
- Protein profiling of the dimorphic pathogenic fungus, Sporothrix schenckii.Mycopathologia · 2012Article
- Sporothrix schenckii and Sporotrichosis.Clinical microbiology reviews · 2011Review
- Calcium/calmodulin kinase1 and its relation to thermotolerance and HSP90 in Sporothrix schenckii: an RNAi and yeast two-hybrid study.BMC microbiology · 2011Article
- Neurospora crassa homologue of Neuronal Calcium Sensor-1 has a role in growth, calcium stress tolerance, and ultraviolet survival.Genetica · 2011Article
- Cytosolic phospholipase A2: a member of the signalling pathway of a new G protein alpha subunit in Sporothrix schenckii.BMC microbiology · 2009Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundSporothrix schenckii is a pathogenic, dimorphic fungus, the etiological agent of sporotrichosis, a subcutaneous lymphatic mycosis. Dimorphism in S. schenckii responds to second messengers such as cAMP and calcium, suggesting the possible involvement of a calcium/calmodulin kinase in its regulation. In this study we describe a novel calcium/calmodulin-dependent protein kinase gene in S. schenckii, sscmk1, and the effects of inhibitors of calmodulin and calcium/calmodulin kinases on the yeast to mycelium transition and the yeast cell cycle.
resultsUsing the PCR homology approach a new member of the calcium/calmodulin kinase family, SSCMK1, was identified in this fungus. The cDNA sequence of sscmk1 revealed an open reading frame of 1,221 nucleotides encoding a 407 amino acid protein with a predicted molecular weight of 45.6 kDa. The genomic sequence of sscmk1 revealed the same ORF interrupted by five introns. Bioinformatic analyses of SSCMK1 showed that this protein had the distinctive features that characterize a calcium/calmodulin protein kinase: a serine/threonine protein kinase domain and a calmodulin-binding domain. When compared to homologues from seven species of filamentous fungi, SSCMK1 showed substantial similarities, except for a large and highly variable region that encompasses positions 330 - 380 of the multiple sequence alignment. Inhibition studies using calmodulin inhibitor W-7, and calcium/calmodulin kinase inhibitors, KN-62 and lavendustin C, were found to inhibit budding by cells induced to re-enter the yeast cell cycle and to favor the yeast to mycelium transition.
conclusionThis study constitutes the first evidence of the presence of a calcium/calmodulin kinase-encoding gene in S. schenckii and its possible involvement as an effector of dimorphism in this fungus. These results suggest that a calcium/calmodulin dependent signaling pathway could be involved in the regulation of dimorphism in this fungus. The results suggest that the calcium/calmodulin kinases of yeasts are evolutionarily distinct from those in filamentous fungi.
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