ArticleMolecular microbiology2008
Periplasmic phosphorylation of lipid A is linked to the synthesis of undecaprenyl phosphate.
Article in Molecular microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 69 papers.
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Who cites it
69 citing papers in PubMed, 124 citations in OpenAlex.
- Direct interaction between phosphotransferase LpxT and ArnT modulates polymyxin B resistance inMicrobiology spectrum · 2026Article
- Analysis of the regulation of undecaprenyl diphosphate dephosphorylation in Escherichia coli.FEBS open bio · 2025Article
- ISmBio · 2024Article
- The Effect of the PhoP/PhoQ System on the Regulation of Multi-Stress Adaptation Induced by Acid Stress inFoods (Basel, Switzerland) · 2024Article
- Real-Time Biosynthetic Reaction Monitoring Informs the Mechanism of Action of Antibiotics.Journal of the American Chemical Society · 2024Article
- Genetic synergy betweenmBio · 2024Article
- Characterizing the role of phosphatidylglycerol-phosphate phosphatases inCell surface (Amsterdam, Netherlands) · 2023Article
- An ST131 clade and a phylogroup A clade bearing an O101-like O-antigen cluster predominate among bloodstreamMicrobial genomics · 2022Article
- Novel small molecules that increase the susceptibility of Neisseria gonorrhoeae to cationic antimicrobial peptides by inhibiting lipid A phosphoethanolamine transferase.The Journal of antimicrobial chemotherapy · 2022Article
- Diacylglycerol Kinase A Is Essential for Polymyxin Resistance Provided by EptA, MCR-1, and Other Lipid A Phosphoethanolamine Transferases.Journal of bacteriology · 2022Article
- Cell Membrane Remodeling Mediates Polymyxin B Resistance inFrontiers in microbiology · 2022Article
- LpxT-Dependent Phosphorylation of Lipid A inFrontiers in microbiology · 2021Article
- Involvement ofAntibiotics (Basel, Switzerland) · 2020Article
- Overexpression ofMicroorganisms · 2020Article
- Metabolic engineering of Escherichia coli to produce a monophosphoryl lipid A adjuvant.Metabolic engineering · 2020Article
- The PhoPQ Two-Component System Is the Major Regulator of Cell Surface Properties, Stress Responses and Plant-Derived Substrate Utilisation During Development ofFrontiers in microbiology · 2020Article
- Loss of the Periplasmic Chaperone Skp and Mutations in the Efflux Pump AcrAB-TolC Play a Role in Acquired Resistance to Antimicrobial Peptides inFrontiers in microbiology · 2020Article
- HupA, the main undecaprenyl pyrophosphate and phosphatidylglycerol phosphate phosphatase in Helicobacter pylori is essential for colonization of the stomach.PLoS pathogens · 2019Article
- Pushing the envelope: LPS modifications and their consequences.Nature reviews. Microbiology · 2019Review
- Discovery ofmSphere · 2018Article
9 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors at 3 institutions in 4 countries.
Funding
Abstract
One-third of the lipid A found in the Escherichia coli outer membrane contains an unsubstituted diphosphate unit at position 1 (lipid A 1-diphosphate). We now report an inner membrane enzyme, LpxT (YeiU), which specifically transfers a phosphate group to lipid A, forming the 1-diphosphate species. (32)P-labelled lipid A obtained from lpxT mutants do not produce lipid A 1-diphosphate. In vitro assays with Kdo(2)-[4'-(32)P]lipid A as the acceptor shows that LpxT uses undecaprenyl pyrophosphate as the substrate donor. Inhibition of lipid A 1-diphosphate formation in wild-type bacteria was demonstrated by sequestering undecaprenyl pyrophosphate with the cyclic polypeptide antibiotic bacitracin, providing evidence that undecaprenyl pyrophosphate serves as the donor substrate within whole bacteria. LpxT-catalysed phosphorylation is dependent upon transport of lipid A across the inner membrane by MsbA, a lipid A flippase, indicating a periplasmic active site. In conclusion, we demonstrate a novel pathway in the periplasmic modification of lipid A that is directly linked to the synthesis of undecaprenyl phosphate, an essential carrier lipid required for the synthesis of various bacterial polymers, such as peptidoglycan.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.