ArticleChromosoma2008
Fluorescence in situ hybridization (FISH) on maize metaphase chromosomes with quantum dot-labeled DNA conjugates.
Article in Chromosoma, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
7 citing papers in PubMed, 42 citations in OpenAlex.
- CRISPR-CISH: an in situ chromogenic DNA repeat detection system for research and life science education.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2025Article
- Developing novel methods to image and visualize 3D genomes.Cell biology and toxicology · 2018Review
- Evaluation of quantum dot conjugated antibodies for immunofluorescent labelling of cellular targets.Beilstein journal of nanotechnology · 2017Article
- Combining M-FISH and Quantum Dot technology for fast chromosomal assignment of transgenic insertions.BMC biotechnology · 2011Article
- Nanotechnology and molecular cytogenetics: the future has not yet arrived.Nano reviews · 2010Article
- Nanomaterial-assisted signal enhancement of hybridization for DNA biosensors: a review.Sensors (Basel, Switzerland) · 2009Article
- Quantum dots as new-generation fluorochromes for FISH: an appraisal.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2009Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Semiconductor nanocrystals, also called quantum dots (QDs), are novel inorganic fluorophores which are brighter and more photostable than organic fluorophores. In the present study, highly dispersive QD-labeled oligonucleotide (TAG)(8) (QD-deoxyribonucleic acid [DNA]) conjugates were constructed via the metal-thiol bond, which can be used as fluorescence in situ hybridization (FISH) probes. FISH analysis of maize metaphase chromosomes using the QD-DNA probes showed that the probes could penetrate maize chromosomes and nuclei and solely hybridized to complementary target DNAs. Compared with the conventional organic dyes such as Cy3 and fluorescein isothiocyanate, this class of luminescent labels bound with oligonucleotides is brighter and more stable against photobleaching on the chromosomes after FISH. These results suggest that QD fluorophores may be a more stable and useful fluorescent label for FISH applications in plant chromosome mapping considering their size-tunable luminescence spectra.
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