ArticleJournal of virology2008
Cell factors stimulate human immunodeficiency virus type 1 reverse transcription in vitro.
Article in Journal of virology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
31 citing papers in PubMed, 44 citations in OpenAlex.
- May I Help You with Your Coat? HIV-1 Capsid Uncoating and Reverse Transcription.International journal of molecular sciences · 2024Review
- Article
- Reconstitution and visualization of HIV-1 capsid-dependent replication and integration in vitro.Science (New York, N.Y.) · 2020Article
- Oxazole-Benzenesulfonamide Derivatives Inhibit HIV-1 Reverse Transcriptase Interaction with Cellular eEF1A and Reduce Viral Replication.Journal of virology · 2019Article
- Article
- Article
- Specific Interaction between eEF1A and HIV RT Is Critical for HIV-1 Reverse Transcription and a Potential Anti-HIV Target.PLoS pathogens · 2015Article
- Binding of the eukaryotic translation elongation factor 1A with the 5'UTR of HIV-1 genomic RNA is important for reverse transcription.Virology journal · 2015Article
- Tetraspanin CD63 is a regulator of HIV-1 replication.International journal of clinical and experimental pathology · 2015Article
- Impairment of HIV-1 cDNA synthesis by DBR1 knockdown.Journal of virology · 2014Article
- Review
- HIV-1-associated PKA acts as a cofactor for genome reverse transcription.Retrovirology · 2013Article
- The unexpected roles of eukaryotic translation elongation factors in RNA virus replication and pathogenesis.Microbiology and molecular biology reviews : MMBR · 2013Review
- Murine leukemia virus glycosylated Gag blocks apolipoprotein B editing complex 3 and cytosolic sensor access to the reverse transcription complex.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- A mutant Tat protein provides strong protection from HIV-1 infection in human CD4+ T cells.Human gene therapy · 2013Article
- Inhibition of reverse transcriptase activity increases stability of the HIV-1 core.Journal of virology · 2013Article
- Eukaryotic elongation factor 1 complex subunits are critical HIV-1 reverse transcription cofactors.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Early events of HIV-1 infection: can signaling be the next therapeutic target?Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology · 2011Review
- A post-entry role for CD63 in early HIV-1 replication.Virology · 2011Article
- Strand transfer and elongation of HIV-1 reverse transcription is facilitated by cell factors in vitro.PloS one · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
After fusion of the human immunodeficiency virus type 1 (HIV-1) envelope with the host cell membrane, the HIV-1 core enters the cell cytoplasm. Core components are then restructured to form the reverse transcription complex (RTC); the biochemical details of this process are currently unclear. To investigate early RTC formation, we characterized the endogenous reverse transcription activity of virions, which was less efficient than reverse transcription during cell infection and suggested a requirement for a cell factor. The addition of detergent to virions released reverse transcriptase and capsid, and reverse transcription products became susceptible to the action of exogenous nucleases, indicating virion disruption. Disruption was coincident with the loss of the endogenous reverse transcription activity of virions, particularly late reverse transcription products. Consistent with this observation, the use of a modified "spin thru" method, which uses brief detergent exposure, also disrupted virions. The addition of lysates made from mammalian cell lines (Jurkat, HEK293T, and NIH 3T3 cells) to virions delipidated by detergent stimulated late reverse transcription efficiency. A complex with reverse transcription activity that was slower sedimenting than virions on a velocity gradient was greatly stimulated to generate full-length reverse transcription products and was associated with only relatively small amounts of capsid. These experiments suggest that cell factors are required for efficient reverse transcription of HIV-1.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.