ArticlePLoS biology2007
Mutation of RNA Pol III subunit rpc2/polr3b Leads to Deficiency of Subunit Rpc11 and disrupts zebrafish digestive development.
Article in PLoS biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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49 citing papers in PubMed, 76 citations in OpenAlex.
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- Multi-Omics Analysis Reveals Differential Molecular Responses of RNA Polymerase Common Subunit ZmRPABC5b for Seedling Development in Maize.Plants (Basel, Switzerland) · 2025Article
- Biallelic variants inBrain communications · 2025Article
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- Molecular basis of neurodegeneration in a mouse model ofbioRxiv : the preprint server for biology · 2024Article
- Novel Pathogenic Variants inHuman mutation · 2024Article
- Hypomyelination, hypodontia and craniofacial abnormalities in a Polr3b mouse model of leukodystrophy.Brain : a journal of neurology · 2023Article
- RNA polymerase common subunit ZmRPABC5b is transcriptionally activated by Opaque2 and essential for endosperm development in maize.Nucleic acids research · 2023Article
- RNA Polymerases I and III in development and disease.Seminars in cell & developmental biology · 2023Review
- The nuclear and cytoplasmic activities of RNA polymerase III, and an evolving transcriptome for surveillance.Nucleic acids research · 2021Article
- Knockdown NRPC2, 3, 8, NRPABC1 and NRPABC2 Affects RNAPIII Activity and Disrupts Seed Development in Arabidopsis.International journal of molecular sciences · 2021Article
- Whole-exome sequencing reveals POLR3B variants associated with progeria-related Wiedemann-Rautenstrauch syndrome.Italian journal of pediatrics · 2021Article
- Zebrafish mutants provide insights into Apolipoprotein B functions during embryonic development and pathological conditions.JCI insight · 2021Article
- RNA Polymerase III Subunit Mutations in Genetic Diseases.Frontiers in molecular biosciences · 2021Review
- Loss of Function of an RNA Polymerase III Subunit Leads to Impaired Maize Kernel Development.Plant physiology · 2020Article
- Analysis of Natural Selection of Immune Genes inFrontiers in genetics · 2020Article
- Brf1 loss and not overexpression disrupts tissues homeostasis in the intestine, liver and pancreas.Cell death and differentiation · 2019Article
- Effects on prostate cancer cells of targeting RNA polymerase III.Nucleic acids research · 2019Article
- Mutation inNeurology. Genetics · 2018Article
- Maf1 and Repression of RNA Polymerase III-Mediated Transcription Drive Adipocyte Differentiation.Cell reports · 2018Article
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7 authors at 3 institutions in 1 country.
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Abstract
The role of RNA polymerase III (Pol III) in developing vertebrates has not been examined. Here, we identify a causative mutation of the second largest Pol III subunit, polr3b, that disrupts digestive organ development in zebrafish slim jim (slj) mutants. The slj mutation is a splice-site substitution that causes deletion of a conserved tract of 41 amino acids in the Polr3b protein. Structural considerations predict that the slj Pol3rb deletion might impair its interaction with Polr3k, the ortholog of an essential yeast Pol III subunit, Rpc11, which promotes RNA cleavage and Pol III recycling. We engineered Schizosaccharomyces pombe to carry an Rpc2 deletion comparable to the slj mutation and found that the Pol III recovered from this rpc2-delta yeast had markedly reduced levels of Rpc11p. Remarkably, overexpression of cDNA encoding the zebrafish rpc11 ortholog, polr3k, rescued the exocrine defects in slj mutants, indicating that the slj phenotype is due to deficiency of Rpc11. These data show that functional interactions between Pol III subunits have been conserved during eukaryotic evolution and support the utility of zebrafish as a model vertebrate for analysis of Pol III function.
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