ArticlePflugers Archiv : European journal of physiology2008
Gating of the shaker potassium channel is modulated differentially by N-glycosylation and sialic acids.
Article in Pflugers Archiv : European journal of physiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 39 citations in OpenAlex.
- Cell Surface Engineering Enables Surfaceome Profiling.ACS chemical biology · 2023Article
- Compromised N-Glycosylation Processing of Kv3.1b Correlates with Perturbed Motor Neuron Structure and Locomotor Activity.Biology · 2021Article
- Wheat germ agglutinin-conjugated fluorescent pH sensors for visualizing proton fluxes.The Journal of general physiology · 2020Article
- N-glycosylation Site Analysis Reveals Sex-related Differences in Protein N-glycosylation in the Rice Brown Planthopper (Molecular & cellular proteomics : MCP · 2020Article
- AThe Journal of general physiology · 2018Article
- Deglycosylation of Shaker KThe Journal of general physiology · 2018Article
- Membrane Distribution and Activity of a Neuronal Voltage-Gated K+ Channel is Modified by Replacement of Complex Type N-Glycans with Hybrid Type.Journal of glycobiology · 2017Article
- Glycosylation of solute carriers: mechanisms and functional consequences.Pflugers Archiv : European journal of physiology · 2016Review
- Neurological aspects of human glycosylation disorders.Annual review of neuroscience · 2015Review
- Sialic acids attached to N- and O-glycans within the Nav1.4 D1S5-S6 linker contribute to channel gating.Biochimica et biophysica acta · 2015Article
- Physiologic and pathophysiologic consequences of altered sialylation and glycosylation on ion channel function.Biochemical and biophysical research communications · 2014Review
- The role of protein N-glycosylation in neural transmission.Glycobiology · 2014Review
- N-glycosylation of TRPM8 ion channels modulates temperature sensitivity of cold thermoreceptor neurons.The Journal of biological chemistry · 2012Article
- DmSAS is required for sialic acid biosynthesis in cultured Drosophila third instar larvae CNS neurons.ACS chemical biology · 2011Article
- Chemical control of metabolically-engineered voltage-gated K+ channels.Bioorganic & medicinal chemistry letters · 2011Article
- Importance of glycosylation on function of a potassium channel in neuroblastoma cells.PloS one · 2011Article
- Sialic acids attached to O-glycans modulate voltage-gated potassium channel gating.The Journal of biological chemistry · 2011Article
- A new genetic model of activity-induced Ras signaling dependent pre-synaptic plasticity in Drosophila.Brain research · 2010Article
- Modulation of the cold-activated cation channel TRPM8 by surface charge screening.The Journal of physiology · 2010Article
- N-Glycosylation regulates fibroblast growth factor receptor/EGL-15 activity in Caenorhabditis elegans in vivo.The Journal of biological chemistry · 2009Article
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Authors and funding
2 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
N-linked glycans, including sialic acids, are integral components of ion channel complexes. To determine if N-linked sugars can modulate a rapidly inactivating K+ channel, the glycosylated Drosophila melanogaster Shaker K+ channel (ShB) and the N-glycosylation-deficient mutant (ShNQ), were studied under conditions of full and reduced sialylation. Through an apparent electrostatic mechanism, full sialylation induced uniform and significant hyperpolarizing shifts in all measured voltage-dependent ShB gating parameters compared to those measured under conditions of reduced sialylation. Steady-state gating of ShNQ was unaffected by changes in sialylation and was nearly identical to that observed for ShB under conditions of reduced sialylation, indicating that N-linked sialic acids were wholly responsible for the observed effects of sialic acid on ShB gating. Interestingly, the rates of transition among channel states and the voltage-independent rates of activation and inactivation were significantly slower for ShNQ compared to ShB. Both effects were independent of sialylation, indicating that N-linked sugars other than sialic acids alter ShB gating kinetics but have little to no effect on the steady-state distribution of channels among states. The effect of sialic acids on channel gating, particularly inactivation gating, and the impact of other N-linked sugars on channel gating kinetics are unique to the ShB isoform. Thus, ShB gating is modulated by two complementary but distinct sugar-dependent mechanisms, (1) an N-linked sialic acid-dependent surface charge effect and (2) a sialic acid-independent effect that is consistent with N-linked sugars affecting the stability of ShB among its functional states.
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