ArticlePflugers Archiv : European journal of physiology2008
Test of blockers of AQP1 water permeability by a high-resolution method: no effects of tetraethylammonium ions or acetazolamide.
Article in Pflugers Archiv : European journal of physiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 54 citations in OpenAlex.
- Epithelial plasma membrane transporters as drug targets.Physiological reviews · 2026Review
- Update on the intriguing roles of AQP4 expression and redistribution in the progression and treatment of glioma.Annals of medicine · 2024Review
- Aquaporin-mediated dysregulation of cell migration in disease states.Cellular and molecular life sciences : CMLS · 2023Review
- Signaling Mechanisms and Pharmacological Modulators Governing Diverse Aquaporin Functions in Human Health and Disease.International journal of molecular sciences · 2022Review
- Identification and characterization of potent and selective aquaporin-3 and aquaporin-7 inhibitors.The Journal of biological chemistry · 2019Article
- Inhibitors of Mammalian Aquaporin Water Channels.International journal of molecular sciences · 2019Review
- Mechanisms of Aquaporin-Facilitated Cancer Invasion and Metastasis.Frontiers in chemistry · 2018Review
- The potential roles of aquaporin 4 in malignant gliomas.Oncotarget · 2017Review
- Aquaporin-Targeted Therapeutics: State-of-the-Field.Advances in experimental medicine and biology · 2017Review
- Fluid and ion transfer across the blood-brain and blood-cerebrospinal fluid barriers; a comparative account of mechanisms and roles.Fluids and barriers of the CNS · 2016Review
- Experimental Evaluation of Proposed Small-Molecule Inhibitors of Water Channel Aquaporin-1.Molecular pharmacology · 2016Article
- Rapid Identification of Novel Inhibitors of the Human Aquaporin-1 Water Channel.Chemical biology & drug design · 2016Article
- Two-dimensional crystal structure of aquaporin-4 bound to the inhibitor acetazolamide.Microscopy (Oxford, England) · 2016Article
- Bumetanide Derivatives AqB007 and AqB011 Selectively Block the Aquaporin-1 Ion Channel Conductance and Slow Cancer Cell Migration.Molecular pharmacology · 2016Article
- Can Stabilization and Inhibition of Aquaporins Contribute to Future Development of Biomimetic Membranes?Membranes · 2015Review
- Aquaporin and brain diseases.Biochimica et biophysica acta · 2014Review
- Aquaporin-1 gene deletion reduces breast tumor growth and lung metastasis in tumor-producing MMTV-PyVT mice.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2014Article
- Acetazolamide attenuates transvascular fluid flux in equine lungs during intense exercise.The Journal of physiology · 2013Article
- Light inactivation of water transport and protein-protein interactions of aquaporin-Killer Red chimeras.The Journal of general physiology · 2012Article
- Aquaporin-1 translocation and degradation mediates the water transportation mechanism of acetazolamide.PloS one · 2012Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The effects of putative water channel blockers were tested on AQP1-expressing Xenopus laevis oocytes by a fast optical method with a time resolution of 1 s and a volume resolution of 20 pl. The oocytes were exposed to external hyposmolarity and the osmotic water permeability (Lp) derived from the initial 10 s of volume change. For longer durations, the effective osmotic gradient across the membrane was reduced significantly because of dilution of the intracellular contents and of ion transport across the membrane. The latter was monitored by voltage clamp of the oocytes. In contrast to previous reports based on slower and less sensitive assays, we found no effects of tetraethylammonium ions (TEA+) and acetazolamide on Lp. We have no single explanation for this, but several factors are considered: (a) If the osmotic gradient is assumed to be constant for periods longer than 10 s, the Lp will be underestimated. (b) Hyposmotic gradients implemented by dilution with water will entail changes in the ionic strength as well; this may enhance loss of salt from the oocyte. (c) By voltage clamping the AQP1-expressing oocytes during hyposmotic challenges, we found that TEA+-treated oocytes were more electrically leaky than untreated ones. This may obscure comparisons between the Lp of treated and untreated oocytes. (d) The nature of the ion transport mechanisms in the plasma membrane depends on how oocytes have been prepared for experiments and on their viability as indicated by the membrane potential. These parameters may vary between laboratories.
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