ArticleEuropean biophysics journal : EBJ2008
Studies of distribution, location and dynamic properties of EGFR on the cell surface measured by image correlation spectroscopy.
Article in European biophysics journal : EBJ, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 38 citations in OpenAlex.
- Spatial and stoichiometric in situ analysis of biomolecular oligomerization at single-protein resolution.Nature communications · 2025Article
- Mathematical modeling of drug-induced receptor internalization in the HER2-positive SKBR3 breast cancer cell-line.Scientific reports · 2019Article
- Characterizing Large-Scale Receptor Clustering on the Single Cell Level: A Comparative Plasmon Coupling and Fluorescence Superresolution Microscopy Study.The journal of physical chemistry. B · 2019Article
- Evaluating the efficacy of the anticancer drug cetuximab by atomic force microscopy.RSC advances · 2018Article
- Development of an image Mean Square Displacement (iMSD)-based method as a novel approach to study the intracellular trafficking of nanoparticles.Acta biomaterialia · 2016Article
- Netrin-1-Regulated Distribution of UNC5B and DCC in Live Cells Revealed by TICCS.Biophysical journal · 2016Article
- Enhanced dimerization drives ligand-independent activity of mutant epidermal growth factor receptor in lung cancer.Molecular biology of the cell · 2015Article
- Cholesterol modulates CFTR confinement in the plasma membrane of primary epithelial cells.Biophysical journal · 2015Article
- Dynamic transition states of ErbB1 phosphorylation predicted by spatial stochastic modeling.Biophysical journal · 2013Article
- Exploring higher-order EGFR oligomerisation and phosphorylation--a combined experimental and theoretical approach.Molecular bioSystems · 2013Article
- Hydrophobic fluorescent probes introduce artifacts into single molecule tracking experiments due to non-specific binding.PloS one · 2013Article
- Epidermal growth factor receptor subunit locations determined in hydrated cells with environmental scanning electron microscopy.Scientific reports · 2013Article
- Mathematical simulation of membrane protein clustering for efficient signal transduction.Annals of biomedical engineering · 2012Review
- Theory and practical recommendations for autocorrelation-based image correlation spectroscopy.Journal of biomedical optics · 2012Article
- Initiation of BMP2 signaling in domains on the plasma membrane.Journal of cellular physiology · 2012Article
- Image correlation spectroscopy of randomly distributed disks.Journal of biological physics · 2011Article
- Distribution of resting and ligand-bound ErbB1 and ErbB2 receptor tyrosine kinases in living cells using number and brightness analysis.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Sorting of EGF and transferrin at the plasma membrane and by cargo-specific signaling to EEA1-enriched endosomes.Journal of cell science · 2008Article
- Quantitative characterization of the large-scale association of ErbB1 and ErbB2 by flow cytometric homo-FRET measurements.Biophysical journal · 2008Article
- Transmembrane helix-helix interactions involved in ErbB receptor signaling.Cell adhesion & migrationReview
Corrections and comments
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Authors and funding
3 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this work, we have studied the distribution and dynamic properties of Epidermal Growth Factor (EGF) receptors in the plasma membrane of fixed and live cells as well as the extent of co-localization of this transmembrane protein with proteins specific for three-membrane microdomains: membrane rafts, caveolae and clathrin-coated pits. This was achieved using a family of image-processing tools called image correlation spectroscopy (ICS), image cross-correlation spectroscopy (ICCS) and dynamic image correlation spectroscopy (DICS). Our results indicate that EGFR is diffusely distributed on the cell surface at 37 degrees C and aggregates as the temperature is lowered to 4 degrees C. This aggregation takes place within 15 min and is reversible. Changes in temperature also affect the diffusion of EGFR by two orders of magnitude. The dynamic properties of EGFR are similar to the dynamic properties of a GPI-anchored protein known to be present in membrane rafts, which motivated us to explore the extent of co-localization of EGFR with this membrane raft protein using ICCS. Our results indicate that more than half of the EGFR population is present in membrane rafts and smaller percentages are present in caveolae and clathrin-coated pits.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.