Evidence map›Paper›PMID 18039824›Full record

ArticleApplied and environmental microbiology2008

Complete genomic sequence of bacteriophage phiEcoM-GJ1, a novel phage that has myovirus morphology and a podovirus-like RNA polymerase.

Nidham Jamalludeen, Andrew M Kropinski, Roger P Johnson, Erika Lingohr, Josée Harel, Carlton L Gyles

Abstract read
In one paragraph

Article in Applied and environmental microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.

0numbers the graph read from it
0cells of the map it votes in
30citing papers in PubMed
2.2field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

30 citing papers in PubMed, 44 citations in OpenAlex.

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  4. Diversity and phage sensitivity to phages of porcine enterotoxigenicApplied and environmental microbiology · 2024
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  6. Genomic Profiling of Non-O157 Shiga ToxigenicPHAGE (New Rochelle, N.Y.) · 2022
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  7. ICTV Virus Taxonomy Profile:The Journal of general virology · 2022
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  8. Review
  9. Article
  10. Article
  11. Article
  12. Article
  13. Complete Genome Sequence of Proteus mirabilis Phage Myduc.Microbiology resource announcements · 2019
    Article
  14. Article
  15. Article
  16. Morphologically DifferentFrontiers in microbiology · 2019
    Article
  17. Article
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 1 country.

Nidham JamalludeenDepartment of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, Ontario N1G 2W1, Canada.
Andrew M Kropinski
Roger P Johnson
Erika Lingohr
Josée Harel
Carlton L Gyles
Public Health Agency of Canada · CAUniversity of Guelph · CAUniversité de Montréal · CA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The complete genome of phiEcoM-GJ1, a lytic phage that attacks porcine enterotoxigenic Escherichia coli of serotype O149:H10:F4, was sequenced and analyzed. The morphology of the phage and the identity of the structural proteins were also determined. The genome consisted of 52,975 bp with a G+C content of 44% and was terminally redundant and circularly permuted. Seventy-five potential open reading frames (ORFs) were identified and annotated, but only 29 possessed homologs. The proteins of five ORFs showed homology with proteins of phages of the family Myoviridae, nine with proteins of phages of the family Podoviridae, and six with proteins of phages of the family Siphoviridae. ORF 1 encoded a T7-like single-subunit RNA polymerase and was preceded by a putative E. coli sigma(70)-like promoter. Nine putative phage promoters were detected throughout the genome. The genome included a tRNA gene of 95 bp that had a putative 18-bp intron. The phage morphology was typical of phages of the family Myoviridae, with an icosahedral head, a neck, and a long contractile tail with tail fibers. The analysis shows that phiEcoM-GJ1 is unique, having the morphology of the Myoviridae, a gene for RNA polymerase, which is characteristic of phages of the T7 group of the Podoviridae, and several genes that encode proteins with homology to proteins of phages of the family Siphoviridae.

Indexed as

Genome, ViralBacteriophagesBase CompositionBase SequenceComputational BiologyDNA-Directed RNA PolymerasesEnterotoxigenic Escherichia coliMicroscopy, ElectronMolecular Sequence DataPodoviridaePolymerase Chain ReactionPotyviridaeSequence Analysis, DNADNA-Directed RNA Polymerases

Identifiers

PMID18039824
PMCPMC2223254
OpenAlexW2105843161

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.