ArticleJournal of biotechnology2008
Exploitation of the S-layer self-assembly system for site directed immobilization of enzymes demonstrated for an extremophilic laminarinase from Pyrococcus furiosus.
Article in Journal of biotechnology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
12 citing papers in PubMed, 56 citations in OpenAlex.
- Protein Assemblies: Nature-Inspired and Designed Nanostructures.Biomacromolecules · 2019Review
- Slp-coated liposomes for drug delivery and biomedical applications: potential and challenges.International journal of nanomedicine · 2019Review
- S-Layer Protein-Based Biosensors.Biosensors · 2018Article
- Biomolecular engineering for nanobio/bionanotechnology.Nano convergence · 2017Review
- Forizymes - functionalised artificial forisomes as a platform for the production and immobilisation of single enzymes and multi-enzyme complexes.Scientific reports · 2016Article
- Ion-specific control of the self-assembly dynamics of a nanostructured protein lattice.ACS nano · 2015Article
- S-layers: principles and applications.FEMS microbiology reviews · 2014Article
- Coal fly ash ceramics: preparation, characterization, and use in the hydrolysis of sucrose.TheScientificWorldJournal · 2014Article
- S-layer homology domain proteins Csac_0678 and Csac_2722 are implicated in plant polysaccharide deconstruction by the extremely thermophilic bacterium Caldicellulosiruptor saccharolyticus.Applied and environmental microbiology · 2012Article
- S-layer fusion proteins--construction principles and applications.Current opinion in biotechnology · 2011Review
- Recombinant glycans on an S-layer self-assembly protein: a new dimension for nanopatterned biomaterials.Small (Weinheim an der Bergstrasse, Germany) · 2008Article
- N-terminal fusion of a hyperthermophilic chitin-binding domain to xylose isomerase from Thermotoga neapolitana enhances kinetics and thermostability of both free and immobilized enzymes.Biotechnology progressArticle
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
A fusion protein based on the S-layer protein SbpA from Bacillus sphaericus CCM 2177 and the enzyme laminarinase (LamA) from Pyrococcus furiosus was designed and overexpressed in Escherichia coli. Due to the construction principle, the S-layer fusion protein fully retained the self-assembly capability of the S-layer moiety, while the catalytic domain of LamA remained exposed at the outer surface of the formed protein lattice. The enzyme activity of the S-layer fusion protein monolayer obtained upon recrystallization on silicon wafers, glass slides and different types of polymer membranes was determined colorimetrically and related to the activity of sole LamA that has been immobilized with conventional techniques. LamA aligned within the S-layer fusion protein lattice in a periodic and orientated fashion catalyzed twice the glucose release from the laminarin polysaccharide substrate in comparison to the randomly immobilized enzyme. In combination with the good shelf-life and the high resistance towards temperature and diverse chemicals, these novel composites are regarded a promising approach for site-directed enzyme immobilization.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.