ArticleBiomaterials2008
Development of a tissue-engineered vascular graft combining a biodegradable scaffold, muscle-derived stem cells and a rotational vacuum seeding technique.
Article in Biomaterials, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
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Who cites it
60 citing papers in PubMed.
- Biomimetic Vascular Scaffold with Seamless Electrospun Aligned Nanofiber Conduit and 3D-Printed Coil Reinforcement.Advanced healthcare materials · 2026Article
- Sacrificial Biofabrication for Vascularization: Concept, Materials, Technologies, and Applications.Advanced materials (Deerfield Beach, Fla.) · 2026Review
- Strong living scaffolds for load-bearing musculoskeletal tissue regeneration.Materials today. Bio · 2025Review
- Endothelial Cells Increase Mesenchymal Stem Cell Differentiation in Scaffold-Free 3D Vascular Tissue.Tissue engineering. Part A · 2025Article
- Development of a Novel Hierarchically Biofabricated Blood Vessel Mimic Decorated with Three Vascular Cell Populations for the Reconstruction of Small-Diameter Arteries.Advanced functional materials · 2024Article
- Cardiovascular Tissue Engineering Models for Atherosclerosis Treatment Development.Bioengineering (Basel, Switzerland) · 2023Review
- Article
- CCL2 loaded microparticles promote acute patency in silk-based vascular grafts implanted in rat aortae.Acta biomaterialia · 2021Article
- Effects of Short-Duration Ethanol Dehydration on Mechanical Properties of Porcine Pericardium.Annals of thoracic and cardiovascular surgery : official journal of the Association of Thoracic and Cardiovascular Surgeons of Asia · 2021Article
- Modifications of the mechanical properties of in vivo tissue-engineered vascular grafts by chemical treatments for a short duration.PloS one · 2021Article
- Slow degrading poly(glycerol sebacate) derivatives improve vascular graft remodeling in a rat carotid artery interposition model.Biomaterials · 2020Article
- Extracellular Vesicles Enhance the Remodeling of Cell-Free Silk Vascular Scaffolds in Rat Aortae.ACS applied materials & interfaces · 2020Article
- Building Scaffolds for Tubular Tissue Engineering.Frontiers in bioengineering and biotechnology · 2020Review
- Development of a Semi-Automated, Bulk Seeding Device for Large Animal Model Implantation of Tissue Engineered Vascular Grafts.Frontiers in bioengineering and biotechnology · 2020Article
- Coaxially-structured fibres with tailored material properties for vascular graft implant.Materials science & engineering. C, Materials for biological applications · 2019Article
- Direct-Write Bioprinting Approach to Construct Multilayer Cellular Tissues.Frontiers in bioengineering and biotechnology · 2019Article
- Assessment of the Angiogenic Potential of 2-Deoxy-D-Ribose Using a NovelFrontiers in bioengineering and biotechnology · 2019Article
- Stem Cell Sources and Graft Material for Vascular Tissue Engineering.Stem cell reviews and reports · 2018Review
- Challenges in vascular tissue engineering for diabetic patients.Acta biomaterialia · 2018Review
- Vascular Tissue Engineering: Progress, Challenges, and Clinical Promise.Cell stem cell · 2018Review
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
There is a clinical need for a tissue-engineered vascular graft (TEVG), and combining stem cells with biodegradable tubular scaffolds appears to be a promising approach. The goal of this study was to characterize the incorporation of muscle-derived stem cells (MDSCs) within tubular poly(ester urethane) urea (PEUU) scaffolds in vitro to understand their interaction, and to evaluate the mechanical properties of the constructs for vascular applications. Porous PEUU scaffolds were seeded with MDSCs using our recently described rotational vacuum seeding device, and cultured inside a spinner flask for 3 or 7 days. Cell viability, number, distribution and phenotype were assessed along with the suture retention strength and uniaxial mechanical behavior of the TEVGs. The seeding device allowed rapid even distribution of cells within the scaffolds. After 3 days, the constructs appeared completely populated with cells that were spread within the polymer. Cells underwent a population doubling of 2.1-fold, with a population doubling time of 35 h. Stem cell antigen-1 (Sca-1) expression by the cells remained high after 7 days in culture (77+/-20% vs. 66+/-6% at day 0) while CD34 expression was reduced (19+/-12% vs. 61+/-10% at day 0) and myosin heavy chain expression was scarce (not quantified). The estimated burst strength of the TEVG constructs was 2127+/-900 mm Hg and suture retention strength was 1.3+/-0.3N. We conclude from this study that MDSCs can be rapidly seeded within porous biodegradable tubular scaffolds while maintaining cell viability and high proliferation rates and without losing stem cell phenotype for up to 7 days of in-vitro culture. The successful integration of these steps is thought necessary to provide rapid availability of TEVGs, which is essential for clinical translation.
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Registered trials
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