ArticleMolecular microbiology2008
Mutation of a single residue, beta-glutamate-20, alters protein-lipid interactions of light harvesting complex II.
Article in Molecular microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 20 citations in OpenAlex.
- Vastly different energy landscapes of the membrane insertions of monomeric gasdermin D and A3.Communications chemistry · 2025Article
- Atomistic simulations reveal impacts of missense mutations on the structure and function of SynGAP1.Briefings in bioinformatics · 2024Article
- Evolutionary engineering and molecular characterization of cobalt-resistantFrontiers in microbiology · 2024Article
- Light harvesting by lamellar chromatophores in Rhodospirillum photometricum.Biophysical journal · 2014Article
- Ca(2+)-binding reduces conformational flexibility of RC-LH1 core complex from thermophile Thermochromatium tepidum.Photosynthesis research · 2012Article
- Analyzing effects of naturally occurring missense mutations.Computational and mathematical methods in medicine · 2012Article
- Specificity of intramembrane protein-lipid interactions.Cold Spring Harbor perspectives in biology · 2011Review
- Influence of K+-dependent membrane lipid composition on the expression of the kdpFABC operon in Escherichia coli.Biochimica et biophysica acta · 2010Article
- Membrane curvature induced by aggregates of LH2s and monomeric LH1s.Biophysical journal · 2009Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
It is well established that assembly of the peripheral antenna complex, LH2, is required for proper photosynthetic membrane biogenesis in the purple bacterium Rhodobacter sphaeroides. The underlying interactions are, as yet, not understood. Here we examined the relationship between the morphology of the photosynthetic membrane and the lipid-protein interactions at the LH2-lipid interface. The non-bilayer lipid, phosphatidylethanolamine, is shown to be highly enriched in the boundary lipid phase of LH2. Sequence alignments indicate a putative lipid binding site, which includes beta-glutamate-20 and the adjacent carotenoid end group. Replacement of beta-glutamate-20 with alanine results in significant reduction of phosphatidylethanolamine and concomitant raise in phosphatidylcholine in the boundary lipid phase of LH2 without altering the lipid composition of the bulk phase. The morphology of the LH2 housing membrane is, however, unaffected by the amino acid replacement. In contrast, simultaneous modification of glutamate-20 and exchange of the carotenoid sphaeroidenone with neurosporene results in significant enlargement of the vesicular membrane invaginations. These findings suggest that the LH2 complex, specifically beta-glutamate-20 and the carotenoids' polar head group, contribute to the shaping of the photosynthetic membrane by specific interactions with surrounding lipid molecules.
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