ArticleThe Journal of physiology2008
Hyperpolarization-activated cyclic nucleotide-modulated 'HCN' channels confer regular and faster rhythmicity to beating mouse embryonic stem cells.
Article in The Journal of physiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Article
- Positive chronotropic action of HCN channel antagonism in human collecting lymphatic vessels.Physiological reports · 2022Article
- TRPC7 regulates the electrophysiological functions of embryonic stem cell-derived cardiomyocytes.Stem cell research & therapy · 2021Article
- HCN2 Channel-Induced Rescue of Brain TeratogenesisFrontiers in cellular neuroscience · 2020Article
- HCN2 Rescues brain defects by enforcing endogenous voltage pre-patterns.Nature communications · 2018Article
- HCN4 ion channel function is required for early events that regulate anatomical left-right patterning in a nodal and lefty asymmetric gene expression-independent manner.Biology open · 2017Article
- TheFrontiers in molecular neuroscience · 2017Article
- High glucose suppresses embryonic stem cell differentiation into cardiomyocytes : High glucose inhibits ES cell cardiogenesis.Stem cell research & therapy · 2016Article
- Introducing a novel mechanism to control heart rate in the ancestral Pacific hagfish.The Journal of experimental biology · 2016Article
- The Interaction between Adult Cardiac Fibroblasts and Embryonic Stem Cell-Derived Cardiomyocytes Leads to Proarrhythmic Changes in In Vitro Cocultures.Stem cells international · 2016Article
- Role of Dynamics in the Autoinhibition and Activation of the Hyperpolarization-activated Cyclic Nucleotide-modulated (HCN) Ion Channels.The Journal of biological chemistry · 2015Article
- Distinct expression patterns of HCN channels in HL-1 cardiomyocytes.BMC cell biology · 2015Article
- A mechanism for the auto-inhibition of hyperpolarization-activated cyclic nucleotide-gated (HCN) channel opening and its relief by cAMP.The Journal of biological chemistry · 2014Article
- Phenotype-dependent role of the L-type calcium current in embryonic stem cell derived cardiomyocytes.American journal of stem cells · 2014Article
- SK4 Ca2+ activated K+ channel is a critical player in cardiac pacemaker derived from human embryonic stem cells.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Asymmetric divergence in structure and function of HCN channel duplicates in Ciona intestinalis.PloS one · 2012Article
- The beginning of the calcium transient in rat embryonic heart.The journal of physiological sciences : JPS · 2011Article
- Transcription profiling of HCN-channel isotypes throughout mouse cardiac development.Basic research in cardiology · 2009Article
- Contribution of pacemaker channels to autonomous electrical activity of differentiated embryonic stem cells.The Journal of physiology · 2008Review
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The hyperpolarization-activated cation current (I(f)), and the hyperpolarization-activated cyclic nucleotide-modulated 'HCN' subunits that underlie it, are important components of spontaneous activity in the embryonic mouse heart, but whether they contribute to this activity in mouse embryonic stem cell-derived cardiomyocytes has not been investigated. We address this issue in spontaneously beating cells derived from mouse embryonic stem cells (mESCs) over the course of development in culture. I(f) and action potentials were recorded from single beating cells at early, intermediate and late development stages using perforated whole-cell voltage- and current-clamp techniques. Our data show that the proportion of cells expressing I(f), and the density of I(f) in these cells, increased during development and correlated with action potential frequency and the rate of diastolic depolarization. The I(f) blocker ZD7288 (0.3 microm) reduced I(f) and the beating rate of embryoid bodies. Taken together, the activation kinetics of I(f) and results from Western blots are consistent with the presence of the HCN2 and HCN3 isoforms. At all stages of development, isoproterenol (isoprenaline) and acetylcholine shifted the voltage dependence of I(f) to more positive and negative voltages, respectively, and they also increased and decreased the beating rate of embryonic cell bodies, respectively. Together, the data suggest that current through HCN2 and HCN3 channels confers regular and faster rhythmicity to mESCs, which mirrors the developing embryonic mouse heart, and contributes to modulation of rhythmicity by autonomic stimulation.
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