ArticleJournal of clinical microbiology2008
Real-time PCR assays of single-nucleotide polymorphisms defining the major Brucella clades.
Article in Journal of clinical microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed, 66 citations in OpenAlex.
- Assays for Identification and Differentiation ofMicroorganisms · 2022Review
- Ecological niche adaptation of Salmonella Typhimurium U288 is associated with altered pathogenicity and reduced zoonotic potential.Communications biology · 2021Article
- Low cost, low tech SNP genotyping tools for resource-limited areas: Plague in Madagascar as a model.PLoS neglected tropical diseases · 2017Article
- Imported human brucellosis in Belgium: Bio and molecular typing of bacterial isolates, 1996-2015.PloS one · 2017Article
- Extended Multilocus Sequence Analysis to Describe the Global Population Structure of the GenusFrontiers in microbiology · 2016Article
- Development and assessment of multiplex high resolution melting assay as a tool for rapid single-tube identification of five Brucella species.BMC research notes · 2014Article
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- Within-host evolution of Brucella canis during a canine brucellosis outbreak in a kennel.BMC veterinary research · 2013Article
- Genotyping of Brucella species using clade specific SNPs.BMC microbiology · 2012Article
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- Nucleotide polymorphism-based single-tube test for robust molecular identification of all currently described Brucella species.Applied and environmental microbiology · 2011Article
- A review of Brucella infection in marine mammals, with special emphasis on Brucella pinnipedialis in the hooded seal (Cystophora cristata).Veterinary research · 2011Review
- High throughput MLVA-16 typing for Brucella based on the microfluidics technology.BMC microbiology · 2011Article
- Characterization of novel Brucella strains originating from wild native rodent species in North Queensland, Australia.Applied and environmental microbiology · 2010Article
- Review of detection of Brucella spp. by polymerase chain reaction.Croatian medical journal · 2010Review
- Rapid identification and discrimination of Brucella isolates by use of real-time PCR and high-resolution melt analysis.Journal of clinical microbiology · 2010Article
- Evaluation of automated and manual commercial DNA extraction methods for recovery of Brucella DNA from suspensions and spiked swabs.Journal of clinical microbiology · 2009Article
- Molecular epidemiology of Brucella genotypes in patients at a major hospital in central Peru.Journal of clinical microbiology · 2009Article
Corrections and comments
- Erratum issued
Authors and funding
10 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Members of the genus Brucella are known worldwide as pathogens of wildlife and livestock and are the most common organisms of zoonotic infection in humans. In general, brucellae exhibit a range of host specificity in animals that has led to the identification of at least seven Brucella species. The genomes of the various Brucella species are highly conserved, which makes the differentiation of species highly challenging. However, we found single-nucleotide polymorphisms (SNPs) in housekeeping and other genes that differentiated the seven main Brucella species or clades and thus enabled us to develop real-time PCR assays based around these SNPs. Screening of a diverse panel of 338 diverse isolates with these assays correctly identified each isolate with its previously determined Brucella clade. Six of the seven clade-specific assays detected DNA concentrations of less than 10 fg, indicating a high level of sensitivity. This SNP-based approach places samples into a phylogenetic framework, allowing reliable comparisons to be made among the lineages of clonal bacteria and providing a solid basis for genotyping. These PCR assays provide a rapid and highly sensitive method of differentiating the major Brucella groups that will be valuable for clinical and forensic applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.