ArticleProceedings of the National Academy of Sciences of the United States of America2007
Pseudouridylation of helix 69 of 23S rRNA is necessary for an effective translation termination.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 60 citations in OpenAlex.
- Compendium of RNA modifications for bacterial stress adaptation.Microbiology and molecular biology reviews : MMBR · 2026Review
- Direct RNA Sequencing Reveals Stress-Dependent and Pathway-Specific rRNA Modification Reprogramming during 50S Biogenesis.Biochemistry · 2026Article
- Direct RNA Sequencing Reveals Stress-Dependent and Pathway-Specific rRNA Modification Reprogramming During 50S Biogenesis.bioRxiv : the preprint server for biology · 2026Article
- Suppressor tRNA in gene therapy.Science China. Life sciences · 2024Review
- Suppressor analysis links trans-translation and ribosomal protein uS7 to RluD function in Escherichia coli.Biochemical and biophysical research communications · 2024Article
- Depletion ofInternational journal of molecular sciences · 2023Article
- Collateral Toxicity Limits the Evolution of Bacterial Release Factor 2 toward Total Omnipotence.Molecular biology and evolution · 2020Article
- Contribution of protein Gar1 to the RNA-guided and RNA-independent rRNA:Ψ-synthase activities of the archaeal Cbf5 protein.Scientific reports · 2018Article
- Random pseuoduridylation in vivo reveals critical region of Escherichia coli 23S rRNA for ribosome assembly.Nucleic acids research · 2017Article
- Contribution of two conserved histidines to the dual activity of archaeal RNA guide-dependent and -independent pseudouridine synthase Cbf5.RNA (New York, N.Y.) · 2015Article
- High-resolution structure of the Escherichia coli ribosome.Nature structural & molecular biology · 2015Article
- Structural and functional insights into the mode of action of a universally conserved Obg GTPase.PLoS biology · 2014Article
- Pseudouridine: still mysterious, but never a fake (uridine)!RNA biology · 2014Review
- Evaluating the reproducibility of quantifying modified nucleosides from ribonucleic acids by LC-UV-MS.Journal of chromatography. B, Analytical technologies in the biomedical and life sciences · 2013Article
- Article
- Impact of methylations of m2G966/m5C967 in 16S rRNA on bacterial fitness and translation initiation.Nucleic acids research · 2012Article
- How much can we learn about the function of bacterial rRNA modification by mining large-scale experimental datasets?Nucleic acids research · 2012Article
- Role of pseudouridine in structural rearrangements of helix 69 during bacterial ribosome assembly.ACS chemical biology · 2012Article
- Base methylations in the double-stranded RNA by a fused methyltransferase bearing unwinding activity.Nucleic acids research · 2012Article
- Comparison of solution conformations and stabilities of modified helix 69 rRNA analogs from bacteria and human.Biopolymers · 2012Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Escherichia coli strains with inactivated rluD genes were previously found to lack the conserved pseudouridines in helix 69 of 23S ribosomal RNA and to grow slowly. A suppressor mutant was isolated with a near normal growth rate that had changed the conserved Glu-172 codon to a Lys codon in prfB, encoding translation termination factor RF2. When nonsense suppression in strains with all combinations of prfB(+)/prfB(E172K) and rluD(+)/rluD::cat was analyzed, misreading of all three stop codons as sense codons was found to be increased by rluD inactivation: Nonsense suppression was increased 2-fold at UAG codons, 9-fold at UAA, and 14-fold at UGA. The increased read-through at UGA corresponds to reading UGA as a sense codon in 30% of the cases. In contrast, the accuracy of reading sense codons appeared unaffected by loss of rluD. When the inactivated rluD gene was combined with the altered prfB, wild-type levels of termination were restored at UAA codons and termination was more efficient than wild type at UGA. These results strongly suggest that at least one of the helix 69 pseudouridines has a function in translation termination. To our knowledge, this is the first described function for a ribosomal RNA pseudouridine modification.
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