ArticleJournal of virology2008
Replication-coupled packaging mechanism in positive-strand RNA viruses: synchronized coexpression of functional multigenome RNA components of an animal and a plant virus in Nicotiana benthamiana cells by agroinfiltration.
Article in Journal of virology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
29 citing papers in PubMed, 61 citations in OpenAlex.
- The curious case of genome packaging and assembly in RNA viruses infecting plants.Frontiers in genetics · 2023Review
- Unravelling the Stability and Capsid Dynamics of the Three Virions of Brome Mosaic Virus Assembled AutonomouslyJournal of virology · 2020Article
- Mapping RNA-capsid interactions and RNA secondary structure within virus particles using next-generation sequencing.Nucleic acids research · 2020Article
- Creating Contacts Between Replication and Movement at Plasmodesmata - A Role for Membrane Contact Sites in Plant Virus Infections?Frontiers in plant science · 2020Review
- Sequence Relationships of RNA Helicases and Other Proteins Encoded by Blunervirus RNAs Highlight Recombinant Evolutionary Origin of Kitaviral Genomes.Frontiers in microbiology · 2020Article
- Packaging of Genomic RNA in Positive-Sense Single-Stranded RNA Viruses: A Complex Story.Viruses · 2019Review
- Laboratory evolution of virus-like nucleocapsids from nonviral protein cages.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- A Functional Link between RNA Replication and Virion Assembly in the PotyvirusJournal of virology · 2018Article
- Viral Delivery of dsRNA for Control of Insect Agricultural Pests and Vectors of Human Disease: Prospects and Challenges.Frontiers in physiology · 2017Review
- The Role of Packaging Sites in Efficient and Specific Virus Assembly.Journal of molecular biology · 2015Article
- Mechanisms of virus assembly.Annual review of physical chemistry · 2015Review
- Nanoparticle encapsidation of Flock house virus by auto assembly of Tobacco mosaic virus coat protein.International journal of molecular sciences · 2014Article
- Pathways for virus assembly around nucleic acids.Journal of molecular biology · 2014Article
- Replication and packaging of Turnip yellow mosaic virus RNA containing Flock house virus RNA1 sequence.BMB reports · 2014Article
- Mutations in the capsid protein of Brome mosaic virus affecting encapsidation eliminate vesicle induction in planta: implications for virus cell-to-cell spread.Journal of virology · 2013Article
- Mutations in the coat protein-binding cis-acting RNA motifs debilitate RNA recombination of Brome mosaic virus.Virus research · 2012Article
- An examination of the electrostatic interactions between the N-terminal tail of the Brome Mosaic Virus coat protein and encapsidated RNAs.Journal of molecular biology · 2012Article
- A physical interaction between viral replicase and capsid protein is required for genome-packaging specificity in an RNA virus.Journal of virology · 2012Article
- Emergence of distinct brome mosaic virus recombinants is determined by the polarity of the inoculum RNA.Journal of virology · 2012Article
- Self-assembly of viral capsid protein and RNA molecules of different sizes: requirement for a specific high protein/RNA mass ratio.Journal of virology · 2012Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Flock house virus (FHV), a bipartite RNA virus of insects and a member of the Nodaviridae family, shares viral replication features with the tripartite brome mosaic virus (BMV), an RNA virus that infects plants and is a member of the Bromoviridae family. In BMV and FHV, genome packaging is coupled to replication, a widely conserved mechanism among positive-strand RNA viruses of diverse origin. To unravel the events that modulate the mechanism of replication-coupled packaging, in this study, we have extended the transfer DNA (T-DNA)-based agroinfiltration system to express functional genome components of FHV in plant cells (Nicotiana benthamiana). Replication, intracellular membrane localization, and packaging characteristics in agroinfiltrated plant cells revealed that T-DNA plasmids of FHV were biologically active and faithfully mimicked complete replication and packaging behavior similar to that observed for insect cells. Synchronized coexpression of wild-type BMV and FHV genome components in plant cells resulted in the assembly of virions packaging the respective viral progeny RNA. To further elucidate the link between replication and packaging, coat protein (CP) open reading frames were precisely exchanged between BMV RNA 3 (B3) and FHV RNA 2 (F2), creating chimeric RNAs expressing heterologous CP genes (B3/FCP and F2/BCP). Coinfiltration of each chimera with its corresponding genome counterpart to provide viral replicase (B1+B2+B3/FCP and F1+F2/BCP) resulted in the expected progeny profiles, but virions exhibited a nonspecific packaging phenotype. Complementation with homologous replicase (with respect to CP) failed to enhance packaging specificity. Taken together, we propose that the transcription of CP mRNA from homologous replication and its translation must be synchronized to confer packaging specificity.
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