ArticleJournal of cellular and molecular medicine
Morphological characterization of very small embryonic-like stem cells (VSELs) by ImageStream system analysis.
Article in Journal of cellular and molecular medicine. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
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Who cites it
54 citing papers in PubMed, 107 citations in OpenAlex.
- Human very small embryonic-like cells generate skeletal structures, in vivo.Stem cells and development · 2013Trial
- Nuclear cholesterol regulates nuclear size and DNA damage responses in cancer stem cells.Neuro-oncology · 2025Article
- Alterations of bovine nucleus pulposus cells with aging.Aging cell · 2023Article
- Endometrial and placental stem cells in successful and pathological pregnancies.Journal of assisted reproduction and genetics · 2023Review
- Proteomic Analysis of Murine Bone Marrow Very Small Embryonic-like Stem Cells at Steady-State Conditions and after In Vivo Stimulation by Nicotinamide and Follicle-Stimulating Factor Reflects their Germ-Lineage Origin and Multi Germ Layer Differentiation Potential.Stem cell reviews and reports · 2023Article
- Cellular enlargement - A new hallmark of aging?Frontiers in cell and developmental biology · 2022Review
- Altered Biology of Testicular VSELs and SSCs by Neonatal Endocrine Disruption Results in Defective Spermatogenesis, Reduced Fertility and Tumor Initiation in Adult Mice.Stem cell reviews and reports · 2020Article
- Hematite Nanoparticles from Unexpected Reaction of Ferrihydrite with Concentrated Acids for Biomedical Applications.Molecules (Basel, Switzerland) · 2020Article
- Megakaryocyte volume modulates bone marrow niche properties and cell migration dynamics.Haematologica · 2020Article
- Identification of Human Very Small Embryonic like Stem Cells (VSELS) in Human Heart Tissue Among Young and Old Individuals.Stem cell reviews and reports · 2020Article
- Article
- Pluripotent Stem Cells in Adult Tissues: Struggling To Be Acknowledged Over Two Decades.Stem cell reviews and reports · 2017Review
- Why are hematopoietic stem cells so 'sexy'? on a search for developmental explanation.Leukemia · 2017Review
- Of Cytometry, Stem Cells and Fountain of Youth.Stem cell reviews and reports · 2017Review
- Mobilization of Peripheral Blood Stem Cells and Changes in the Concentration of Plasma Factors Influencing their Movement in Patients with Panic Disorder.Stem cell reviews and reports · 2017Article
- A Novel View of the Adult Stem Cell Compartment From the Perspective of a Quiescent Population of Very Small Embryonic-Like Stem Cells.Circulation research · 2017Review
- Empowering Adult Stem Cells for Myocardial Regeneration V2.0: Success in Small Steps.Circulation research · 2016Review
- Migration of Bone Marrow-Derived Very Small Embryonic-Like Stem Cells toward An Injured Spinal Cord.Cell journal · 2016Article
- Novel Action of FSH on Stem Cells in Adult Mammalian Ovary Induces Postnatal Oogenesis and Primordial Follicle Assembly.Stem cells international · 2016Review
- Identification of New Rat Bone Marrow-Derived Population of Very Small Stem Cell with Oct-4A and Nanog Expression by Flow Cytometric Platforms.Stem cells international · 2016Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
Recently, our group purified a rare population of primitive Sca1(+)/Lin(-)/CD45(-) cells from murine bone marrow by employing multiparameter cell sorting. Based on flow cytometric and gene expression analysis, these cells have been shown to express several markers of embryonic stem cells and were accordingly termed Very Small Embryonic-Like stem cells (VSELs). In order to better characterize VSELs, we focused on their morphological parameters (e.g. diameter, nuclear to cytoplasmic ratio, cytoplasmic area) as well as expression of Oct-4. To examine the morphological features of VSELs, we employed a multi-dimensional approach, including (i) traditional flow cytometry, (ii) a novel approach, which is ImageStream (IS) cytometry and (iii) confocal microscopy. We demonstrate by all of the sensitive and precise methods employed, that VSELs are a population of very small cells, which are significantly smaller than haematopoetic stem cells (HSC) (3.63 +/- 0.09 versus 6.54 +/-0.17 microm in diameter). They also exhibit higher nuclear to cytoplasmic ratio and lower cytoplasmic area as compared with HSCs and mature granulocytes. Besides confirming the size characteristics, confocal microscopic analysis also confirmed that VSELs express Oct-4, a marker of pluripotent embryonic stem cells. Morphological examination reveals that VSELs are unusually small eukaryotic cells that posses several characteristics of embryonic cells. Thus, FACS-based sorting strategies should consider that adult tissues harbour small primitive cells that are larger than platelets and smaller than erythrocytes.
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Registered trials
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