ArticleMicrovascular research2008
PECAM-1 isoform-specific functions in PECAM-1-deficient brain microvascular endothelial cells.
Article in Microvascular research, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 26 citations in OpenAlex.
- Alternative splicing in endothelial cells: novel therapeutic opportunities in cancer angiogenesis.Journal of experimental & clinical cancer research : CR · 2020Review
- Bcl-2 Expression in Pericytes and Astrocytes Impacts Vascular Development and Homeostasis.Scientific reports · 2019Article
- PEDF expression affects the oxidative and inflammatory state of choroidal endothelial cells.American journal of physiology. Cell physiology · 2018Article
- Article
- Mice dental pulp and periodontal ligament endothelial cells exhibit different proangiogenic properties.Tissue & cell · 2018Article
- Platelet Endothelial Cell Adhesion Molecule-1 and Oligodendrogenesis: Significance in Alcohol Use Disorders.Brain sciences · 2017Review
- PEDF expression affects retinal endothelial cell proangiogenic properties through alterations in cell adhesive mechanisms.American journal of physiology. Cell physiology · 2017Article
- High glucose promotes the migration of retinal pigment epithelial cells through increased oxidative stress and PEDF expression.American journal of physiology. Cell physiology · 2016Article
- Factors controlling permeability of the blood-brain barrier.Cellular and molecular life sciences : CMLS · 2016Review
- PECAM-1 isoforms, eNOS and endoglin axis in regulation of angiogenesis.Clinical science (London, England : 1979) · 2015Review
- Expression of pigment epithelium-derived factor and thrombospondin-1 regulate proliferation and migration of retinal pigment epithelial cells.Physiological reports · 2015Article
- PECAM-1: regulator of endothelial junctional integrity.Cell and tissue research · 2014Review
- Lack of Cyp1b1 promotes the proliferative and migratory phenotype of perivascular supporting cells.Laboratory investigation; a journal of technical methods and pathology · 2013Article
- Aberrant production of extracellular matrix proteins and dysfunction in kidney endothelial cells with a short duration of diabetes.American journal of physiology. Renal physiology · 2013Article
- PECAM-1 regulates proangiogenic properties of endothelial cells through modulation of cell-cell and cell-matrix interactions.American journal of physiology. Cell physiology · 2010Article
- Opposing effects of bim and bcl-2 on lung endothelial cell migration.American journal of physiology. Lung cellular and molecular physiology · 2010Article
- PECAM-1: conflicts of interest in inflammation.Life sciences · 2010Review
- Myricetin inhibits UVB-induced angiogenesis by regulating PI-3 kinase in vivo.Carcinogenesis · 2010Article
- Evaluation of lymphangiogenesis in acellular dermal matrix.Indian journal of plastic surgery : official publication of the Association of Plastic Surgeons of IndiaArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Platelet endothelial cell adhesion molecule-1 (PECAM-1) is alternatively spliced generating eight isoforms that only differ in the length of their cytoplasmic domain. Multiple isoforms of PECAM-1 are present in the endothelium and their expression levels are regulated during vascular development and angiogenesis. However, the functional significance of PECAM-1 isoforms during these processes remains largely unknown. We recently showed that mouse brain endothelial (bEND) cells prepared from PECAM-1-deficient (PECAM-1-/-) mice differ in their cell adhesive and migratory properties compared to PECAM-1+/+ bEND cells. Here we demonstrate that the restoration of PECAM-1 expression in these cells affects their adhesive and migratory properties in an isoform-specific manner. Expression of Delta14&15 PECAM-1, the predominant isoform present in the mouse endothelium, in PECAM-1-/- bEND cells activated MAPK/ERKs, disrupted adherens junctions, and enhanced cell migration and capillary morphogenesis in Matrigel. In contrast, expression of Delta15 PECAM-1 in PECAM-1-/- bEND cells had minimal effects on their activation of MAPK/ERKs, migration, and capillary morphogenesis. The effects of PECAM-1 on cell adhesive and migratory properties were mediated in an isoform-specific manner, at least in part, through its interactions with intracellular signaling proteins, including SHP-2 and Src. These results suggest that the impact of PECAM-1 on EC adhesion, migration, and capillary morphogenesis is modulated by alternative splicing of its cytoplasmic domain.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.