ArticleMolecular genetics and metabolism2008
Global metabolic effects of glycerol kinase overexpression in rat hepatoma cells.
Article in Molecular genetics and metabolism, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 44 citations in OpenAlex.
- Experimental validation of cuproptosis-associated molecular signatures and their immunological implications in pulmonary tuberculosis.Frontiers in immunology · 2025Article
- Transcription profiling reveals tissue-specific metabolic pathways in the fat body and ovary of the diapausing mosquito Culex pipiens.Comparative biochemistry and physiology. Part D, Genomics & proteomics · 2024Article
- Exploring a Novel Role of Glycerol Kinase 1 in Prostate Cancer PC-3 Cells.Biomolecules · 2024Article
- Glycerol kinase enzyme is a prognostic predictor in esophageal carcinoma and is associated with immune cell infiltration.Scientific reports · 2024Article
- Genome wide transcriptome analysis provides bases on hepatic lipid metabolism disorder affected by increased dietary grain ratio in fattening lambs.BMC genomics · 2023Article
- Isotope-Assisted Metabolic Flux Analysis: A Powerful Technique to Gain New Insights into the Human Metabolome in Health and Disease.Metabolites · 2022Review
- Recent findings in the regulation of G6PD and its role in diseases.Frontiers in pharmacology · 2022Review
- Hepatic Glycerol Metabolism-Related Genes in Carnivorous Rainbow Trout (Frontiers in physiology · 2020Article
- Oncogenic KRAS Induces NIX-Mediated Mitophagy to Promote Pancreatic Cancer.Cancer discovery · 2019Article
- The challenge and potential of photosynthesis: unique considerations for metabolic flux measurements in photosynthetic microorganisms.Biotechnology letters · 2019Review
- Experimental evidence and isotopomer analysis of mixotrophic glucose metabolism in the marine diatom Phaeodactylum tricornutum.Microbial cell factories · 2013Article
- Metabolic analyses elucidate non-trivial gene targets for amplifying dihydroartemisinic acid production in yeast.Frontiers in microbiology · 2013Article
- Isotopically nonstationary 13C flux analysis of Myc-induced metabolic reprogramming in B-cells.Metabolic engineering · 2013Article
- Genotype-specific responses in Atlantic salmon (Salmo salar) subject to dietary fish oil replacement by vegetable oil: a liver transcriptomic analysis.BMC genomics · 2011Article
- Moonlighting function of glycerol kinase causes systems-level changes in rat hepatoma cells.Metabolic engineering · 2010Article
- Fenofibrate simultaneously induces hepatic fatty acid oxidation, synthesis, and elongation in mice.The Journal of biological chemistry · 2009Article
- Evaluation of 13C isotopic tracers for metabolic flux analysis in mammalian cells.Journal of biotechnology · 2009Article
- Transcriptomic and network component analysis of glycerol kinase in skeletal muscle using a mouse model of glycerol kinase deficiency.Molecular genetics and metabolism · 2009Article
- Active and inactive metabolic pathways in tumor spheroids: determination by GC-MS.Biotechnology progressArticle
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Glycerol kinase has several diverse activities in mammalian cells. Glycerol kinase deficiency is a complex, single-gene, inborn error of metabolism wherein no genotype-phenotype correlation has been established. Since glycerol kinase has been suggested to exhibit additional activities than glycerol phosphorylation, expression level perturbation in this enzyme may affect cellular physiology globally. To investigate this possibility, we conducted metabolic investigations of wild-type and two glycerol kinase-overexpressing H4IIE rat hepatoma cell lines constructed in this study. The glycerol kinase-overexpressing cell lines exhibited a significantly higher consumption of carbon sources per cell, suggesting excess carbon expenditure. Furthermore, we quantified intracellular metabolic fluxes by employing stable isotope 13C labeling with a mathematically designed substrate mixture, gas chromatography-mass spectrometry, and comprehensive isotopomer balancing. This flux analysis revealed that the pentose phosphate pathway flux in the glycerol kinase-overexpressing cell lines was 2-fold higher than that in the wild-type, in addition to subtler flux changes in other pathways of carbohydrate metabolism. Furthermore, the activity and transcript level of the lipogenic enzyme glucose-6-phosphate dehydrogenase, the rate-limiting enzyme of the pentose phosphate pathway, were also about 2-fold higher than that of the wild-type; these data corroborate the flux analysis results. This study shows that glycerol kinase affects carbon metabolism globally, possibly through its additional functions, and highlights glycerol kinase's multifaceted role in cellular physiology.
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