ArticleMolecular microbiology2007
Subcellular location characteristics of the Pseudomonas aeruginosa GGDEF protein, WspR, indicate that it produces cyclic-di-GMP in response to growth on surfaces.
Article in Molecular microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 140 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
140 citing papers in PubMed, 247 citations in OpenAlex.
- Distinct roles of PilI and ChpC inApplied and environmental microbiology · 2026Article
- Cyclic di-GMP directly reprograms the multidrug resistance machinery via UspG-mediated sequestration of RamR in Klebsiella.PLoS pathogens · 2026Article
- c-di-GMP-mediated pause behavior enablesApplied and environmental microbiology · 2026Article
- UnderstandingMicroorganisms · 2026Review
- Transcriptional profiling ofFrontiers in microbiology · 2026Article
- Identification of key targets driving biofilm formation and virtual screening of potential inhibitors inFrontiers in microbiology · 2026Article
- Article
- Integrated genomic and mutagenesis analysis of Pseudomonas argentinensis SA190 reveals mechanisms of plant root colonization and stress resilience.BMC microbiology · 2025Article
- Tetracycline inducesApplied and environmental microbiology · 2025Article
- Interspecies surfactants serve as public goods enabling surface motility inJournal of bacteriology · 2024Article
- ThemSphere · 2024Article
- Article
- Interspecies surfactants serve as public goods enabling surface motility inbioRxiv : the preprint server for biology · 2024Article
- FlhF affects the subcellular clustering of WspR through HsbR inApplied and environmental microbiology · 2024Article
- Unraveling the molecular dynamics ofFuture microbiology · 2024Article
- Biofilm formation: mechanistic insights and therapeutic targets.Molecular biomedicine · 2023Review
- Nucleotides as Bacterial Second Messengers.Molecules (Basel, Switzerland) · 2023Review
- The non-attached biofilm aggregate.Communications biology · 2023Review
- The role of RNA regulators, quorum sensing and c-di-GMP in bacterial biofilm formation.FEBS open bio · 2023Review
- Polarity of c-di-GMP synthesis and degradation.microLife · 2023Review
80 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The Pseudomonas aeruginosa Wsp signal transduction system produces cyclic-di-GMP (c-di-GMP), an intracellular messenger that stimulates biofilm formation and suppresses motility. The Wsp system is homologous to chemotaxis systems and includes a membrane-bound receptor protein, WspA, and a response regulator GGDEF protein, WspR, that catalyses c-di-GMP synthesis when phosphorylated. We found that the subcellular distributions of fluorescent protein-tagged WspA and WspR differed markedly from their chemotaxis counterparts. WspA-YFP formed patches in cells whereas WspR-YFP was dispersed when unphosphorylated and formed bright cytoplasmic clusters when phosphorylated. WspR formed clusters in cells of a DeltawspF mutant, a genetic background that causes constitutive phosphorylation of WspR, but was dispersed in cells of a wspA mutant, a genetic background necessary for WspR phosphorylation. In addition, WspR mutated at Asp70, its predicted site of phosphorylation, did not form clusters. C-di-GMP synthesis was not required for cluster formation. WspR-YFP was dispersed in liquid-grown wild-type cells, but formed clusters that sometimes appeared and disappeared over the course of a few minutes in cells grown on an agar surface. Our results suggest that the compartmentalized production of c-di-GMP in response to a stimulus associated with growth on a surface is an important functional characteristic of the Wsp system.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.