ArticleJournal of biomolecular NMR2008
High-resolution structure determination of the CylR2 homodimer using paramagnetic relaxation enhancement and structure-based prediction of molecular alignment.
Article in Journal of biomolecular NMR, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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21 citing papers in PubMed, 32 citations in OpenAlex.
- Reconstruction of Coupled Intra- and Interdomain Protein Motion from Nuclear and Electron Magnetic Resonance.Journal of the American Chemical Society · 2021Article
- Long-range paramagnetic NMR data can provide a closer look on metal coordination in metalloproteins.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2018Article
- The dynamic dimer structure of the chaperone Trigger Factor.Nature communications · 2017Article
- The ClusPro web server for protein-protein docking.Nature protocols · 2017Article
- Visualizing transient dark states by NMR spectroscopy.Quarterly reviews of biophysics · 2015Article
- Virulence Plasmids of Nonsporulating Gram-Positive Pathogens.Microbiology spectrum · 2014Review
- Applications of NMR-based PRE and EPR-based DEER spectroscopy to homodimer chain exchange characterization and structure determination.Methods in molecular biology (Clifton, N.J.) · 2014Article
- Advances in NMR structures of integral membrane proteins.Current opinion in structural biology · 2013Review
- DOT2: Macromolecular docking with improved biophysical models.Journal of computational chemistry · 2013Article
- Measurement of rate constants for homodimer subunit exchange using double electron-electron resonance and paramagnetic relaxation enhancements.Journal of biomolecular NMR · 2013Article
- Convenient method for resolving degeneracies due to symmetry of the magnetic susceptibility tensor and its application to pseudo contact shift-based protein-protein complex structure determination.Journal of biomolecular NMR · 2012Article
- Structural NMR of protein oligomers using hybrid methods.Journal of structural biology · 2011Review
- Integrated analysis of the conformation of a protein-linked spin label by crystallography, EPR and NMR spectroscopy.Journal of biomolecular NMR · 2011Article
- Rapid exploration of the folding topology of helical membrane proteins using paramagnetic perturbation.Protein science : a publication of the Protein Society · 2010Article
- Combining NMR and EPR methods for homodimer protein structure determination.Journal of the American Chemical Society · 2010Article
- Theory, practice, and applications of paramagnetic relaxation enhancement for the characterization of transient low-population states of biological macromolecules and their complexes.Chemical reviews · 2009Review
- Automated NMR Assignment and Protein Structure Determination using Sparse Dipolar Coupling Constraints.Progress in nuclear magnetic resonance spectroscopy · 2009Article
- Two-point anchoring of a lanthanide-binding peptide to a target protein enhances the paramagnetic anisotropic effect.Journal of biomolecular NMR · 2009Article
- Outcome of a workshop on applications of protein models in biomedical research.Structure (London, England : 1993) · 2009Article
- Assignment-free solution NMR method reveals CesT as an unswapped homodimer.Protein science : a publication of the Protein Society · 2008Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Structure determination of homooligomeric proteins by NMR spectroscopy is difficult due to the lack of chemical shift perturbation data, which is very effective in restricting the binding interface in heterooligomeric systems, and the difficulty of obtaining a sufficient number of intermonomer distance restraints. Here we solved the high-resolution solution structure of the 15.4 kDa homodimer CylR2, the regulator of cytolysin production from Enterococcus faecalis, which deviates by 1.1 angstroms from the previously determined X-ray structure. We studied the influence of different experimental information such as long-range distances derived from paramagnetic relaxation enhancement, residual dipolar couplings, symmetry restraints and intermonomer Nuclear Overhauser Effect restraints on the accuracy of the derived structure. In addition, we show that it is useful to combine experimental information with methods of ab initio docking when the available experimental data are not sufficient to obtain convergence to the correct homodimeric structure. In particular, intermonomer distances may not be required when residual dipolar couplings are compared to values predicted on the basis of the charge distribution and the shape of ab initio docking solutions.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.