ArticlePflugers Archiv : European journal of physiology2008
Slow spontaneous [Ca2+] i oscillations reflect nucleotide release from renal epithelia.
Article in Pflugers Archiv : European journal of physiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 53 citations in OpenAlex.
- GPER1 activation regulates renal purinergic P2YAmerican journal of physiology. Regulatory, integrative and comparative physiology · 2026Article
- Ion channels as a therapeutic target for renal fibrosis.Frontiers in physiology · 2022Review
- Recent advances in understanding ion transport mechanisms in polycystic kidney disease.Clinical science (London, England : 1979) · 2021Review
- Extracellular Nucleotides and P2 Receptors in Renal Function.Physiological reviews · 2020Review
- Calcium Oscillatory Behavior and Its Possible Role during Wound Healing in Bovine Corneal Endothelial Cells in Culture.BioMed research international · 2019Article
- Characterization of purinergic receptor expression in ARPKD cystic epithelia.Purinergic signalling · 2018Article
- Role of renal TRP channels in physiology and pathology.Seminars in immunopathology · 2016Review
- [Ca2+]i Oscillations and IL-6 Release Induced by α-Hemolysin from Escherichia coli Require P2 Receptor Activation in Renal Epithelia.The Journal of biological chemistry · 2015Article
- Deciphering physiological role of the mechanosensitive TRPV4 channel in the distal nephron.American journal of physiology. Renal physiology · 2015Review
- Reactive oxygen species as important determinants of medullary flow, sodium excretion, and hypertension.American journal of physiology. Renal physiology · 2015Review
- P2X receptors trigger intracellular alkalization in isolated perfused mouse medullary thick ascending limb.Acta physiologica (Oxford, England) · 2015Article
- Purinergic signalling in the kidney in health and disease.Purinergic signalling · 2014Review
- TRPV4 dysfunction promotes renal cystogenesis in autosomal recessive polycystic kidney disease.Journal of the American Society of Nephrology : JASN · 2013Article
- Novel insights into TRPV4 function in the kidney.Pflugers Archiv : European journal of physiology · 2013Review
- Renal epithelial cells can release ATP by vesicular fusion.Frontiers in physiology · 2013Article
- Medullary thick ascending limb buffer vasoconstriction of renal outer-medullary vasa recta in salt-resistant but not salt-sensitive rats.Hypertension (Dallas, Tex. : 1979) · 2012Article
- The touching story of purinergic signaling in epithelial and endothelial cells.Purinergic signalling · 2012Review
- Characteristics of spontaneous calcium oscillations in renal tubular epithelial cells.Clinical and experimental nephrology · 2012Article
- P2Y receptors and kidney function.Wiley interdisciplinary reviews. Membrane transport and signaling · 2012Article
- Shear-induced volume decrease in MDCK cells.Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology · 2012Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Renal epithelia can be provoked mechanically to release nucleotides, which subsequently increases the intracellular Ca(2+) concentration [Ca(2+)](i) through activation of purinergic (P2) receptors. Cultured cells often show spontaneous [Ca(2+)](i) oscillations, a feature suggested to involve nucleotide signalling. In this study, fluo-4 loaded Madin-Darby canine kidney (MDCK) cells are used as a model for quantification and characterisation of spontaneous [Ca(2+)](i) increases in renal epithelia. Spontaneous [Ca(2+)](i) increases occurred randomly as single cell events. During an observation period of 1 min, 10.9 +/- 6.7% (n = 23) of the cells showed spontaneous [Ca(2+)](i) increases. Spontaneous adenosine triphosphate (ATP) release from MDCK cells was detected directly by luciferin/luciferase. Scavenging of ATP by apyrase or hexokinase markedly reduced the [Ca(2+)](i) oscillatory activity, whereas inhibition of ecto-ATPases (ARL67156) enhanced the [Ca(2+)](i) oscillatory activity. The association between spontaneous [Ca(2+)](i) increases and nucleotide signalling was further tested in 132-1N1 cells lacking P2 receptors. These cells hardly showed any spontaneous [Ca(2+)](i) increases. Transfection with either hP2Y(6) or hP2Y(2) receptors revealed a striking degree of oscillations. Similar spontaneous [Ca(2+)](i) increases were observed in freshly isolated, perfused mouse medullary thick ascending limb (mTAL). The oscillatory activity was reduced by basolateral apyrase and substantially lower in mTAL from P2Y(2) knock out mice (0.050 +/- 0.020 events per second, n = 8) compared to the wild type (0.147 +/- 0.018 events per second, n = 9). These findings indicate that renal epithelia spontaneously release nucleotides leading to P2-receptor-dependent [Ca(2+)](i) oscillations. Thus, tonic nucleotide release is likely to modify steady state renal function.
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