ArticleThe Journal of cell biology2007
Nuclear fusion during yeast mating occurs by a three-step pathway.
Article in The Journal of cell biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 28 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
28 citing papers in PubMed, 46 citations in OpenAlex.
- The influence of cell-cell fusion on cancer heterogeneity and its promise for targeted treatments.Cellular oncology (Dordrecht, Netherlands) · 2026Review
- Nuclear Fusion in Yeast and Plant Reproduction.Plants (Basel, Switzerland) · 2023Article
- Article
- Pcp1/pericentrin controls the SPB number in fission yeast meiosis and ploidy homeostasis.The Journal of cell biology · 2022Article
- C. elegans pronuclei fuse after fertilization through a novel membrane structure.The Journal of cell biology · 2020Article
- Membrane fusion drives pronuclear meeting in the one-cell embryo.The Journal of cell biology · 2020Article
- Mechanics of nuclear membranes.Journal of cell science · 2019Review
- Article
- Influence of the bud neck on nuclear envelope fission in Saccharomyces cerevisiae.Experimental cell research · 2017Article
- Characterization of spindle pole body duplication reveals a regulatory role for nuclear pore complexes.The Journal of cell biology · 2017Article
- Mesenchymal stem cells generate distinct functional hybrids in vitro via cell fusion or entosis.Scientific reports · 2016Article
- The Malleable Nature of the Budding Yeast Nuclear Envelope: Flares, Fusion, and Fenestrations.Journal of cellular physiology · 2016Review
- Caenorhabditis elegans polo-like kinase PLK-1 is required for merging parental genomes into a single nucleus.Molecular biology of the cell · 2015Article
- Electron microscopy for ultrastructural analysis and protein localization inMicrobial cell (Graz, Austria) · 2015Review
- Cell biology of yeast zygotes, from genesis to budding.Biochimica et biophysica acta · 2015Review
- Kar5p is required for multiple functions in both inner and outer nuclear envelope fusion in Saccharomyces cerevisiae.G3 (Bethesda, Md.) · 2014Article
- Breaching the nuclear envelope in development and disease.The Journal of cell biology · 2014Review
- ER-associated SNAREs and Sey1p mediate nuclear fusion at two distinct steps during yeast mating.Molecular biology of the cell · 2013Article
- Spindle pole body-anchored Kar3 drives the nucleus along microtubules from another nucleus in preparation for nuclear fusion during yeast karyogamy.Genes & development · 2013Article
- Septin-containing barriers control the differential inheritance of cytoplasmic elements.Cell reports · 2013Article
Corrections and comments
- Erratum issued
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
In Saccharomyces cerevisiae, mating culminates in nuclear fusion to produce a diploid zygote. Two models for nuclear fusion have been proposed: a one-step model in which the outer and inner nuclear membranes and the spindle pole bodies (SPBs) fuse simultaneously and a three-step model in which the three events occur separately. To differentiate between these models, we used electron tomography and time-lapse light microscopy of early stage wild-type zygotes. We observe two distinct SPBs in approximately 80% of zygotes that contain fused nuclei, whereas we only see fused or partially fused SPBs in zygotes in which the site of nuclear envelope (NE) fusion is already dilated. This demonstrates that SPB fusion occurs after NE fusion. Time-lapse microscopy of zygotes containing fluorescent protein tags that localize to either the NE lumen or the nucleoplasm demonstrates that outer membrane fusion precedes inner membrane fusion. We conclude that nuclear fusion occurs by a three-step pathway.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.