ArticleInfection and immunity2008
Contribution of the SitABCD, MntH, and FeoB metal transporters to the virulence of avian pathogenic Escherichia coli O78 strain chi7122.
Article in Infection and immunity, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
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62 citing papers in PubMed, 102 citations in OpenAlex.
- Distribution and Co-Occurrence of Selected Virulence-Associated Genes inPathogens (Basel, Switzerland) · 2026Article
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- Orchestrating infection: the impact of RyfA and TimR sRNAs on stress resistance and virulence in avian pathogenicApplied and environmental microbiology · 2026Article
- MappingAntibiotics (Basel, Switzerland) · 2026Article
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- The dual implications of iron homeostasis as a bacterial threat: its roles in virulence and antimicrobial resistance.Archives of microbiology · 2025Review
- Insights into the phylogenetic profile and virulence-associated genes of multidrug-resistant uropathogenicIJID regions · 2025Article
- Determinants of bacterial survival and proliferation in blood.FEMS microbiology reviews · 2024Review
- Emerging roles for ABC transporters as virulence factors in uropathogenicProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Versatile allelic replacement and self-excising integrative vectors for plasmid genome mutation and complementation.Microbiology spectrum · 2024Article
- Genotypic Antimicrobial Resistance Profiles of DiarrheagenicInfection and drug resistance · 2024Article
- Functional Characterization of FeoAB in Iron Acquisition and Pathogenicity in Riemerella anatipestifer.Microbiology spectrum · 2023Article
- Epistatic interactions between the high pathogenicity island and other iron uptake systems shape Escherichia coli extra-intestinal virulence.Nature communications · 2023Article
- The Diversity ofMicroorganisms · 2023Review
- A general protocol for the expression and purification of the intact transmembrane transporter FeoB.Biochimica et biophysica acta. Biomembranes · 2022Article
- Genomic Analysis ofMicroorganisms · 2022Article
- Characteristics, pathogenic mechanism, zoonotic potential, drug resistance, and prevention of avian pathogenicFrontiers in microbiology · 2022Review
- Regulation and distinct physiological roles of manganese in bacteria.FEMS microbiology reviews · 2021Review
- Molecular Mechanism of Nramp-Family Transition Metal Transport.Journal of molecular biology · 2021Review
- The RyfA small RNA regulates oxidative and osmotic stress responses and virulence in uropathogenic Escherichia coli.PLoS pathogens · 2021Article
2 more citing papers are in PubMed but not listed here.
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Authors and funding
8 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The roles of SitABCD, MntH, and FeoB metal transporters in the virulence of avian pathogenic Escherichia coli (APEC) O78 strain chi7122 were assessed using isogenic mutants in chicken infection models. In a single-strain infection model, compared to chi7122, the Deltasit strain demonstrated reduced colonization of the lungs, liver, and spleen. Complementation of the Deltasit strain restored virulence. In a coinfection model, compared to the virulent APEC strain, the Deltasit strain demonstrated mean 50-fold, 126-fold, and 25-fold decreases in colonization of the lungs, liver, and spleen, respectively. A DeltamntH Deltasit strain was further attenuated, demonstrating reduced persistence in blood and mean 1,400-fold, 954-fold, and 83-fold reduced colonization in the lungs, liver, and spleen, respectively. In coinfections, the DeltafeoB Deltasit strain demonstrated reduced persistence in blood but increased colonization of the liver. The DeltamntH, DeltafeoB, and DeltafeoB DeltamntH strains were as virulent as the wild type in either of the infection models. Strains were also tested for sensitivity to oxidative stress-generating agents. The DeltamntH Deltasit strain was the most sensitive strain and was significantly more sensitive than the other strains to hydrogen peroxide, plumbagin, and paraquat. sit sequences were highly associated with APEC and human extraintestinal pathogenic E. coli compared to commensal isolates and diarrheagenic E. coli. Comparative genomic analyses also demonstrated that sit sequences are carried on conjugative plasmids or associated with phage elements and were likely acquired by distinct genetic events among pathogenic E. coli and Shigella sp. strains. Overall, the results demonstrate that SitABCD contributes to virulence and, together with MntH, to increased resistance to oxidative stress.
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