ArticleNucleic acids research2008
Identification of functional domains in Arabidopsis thaliana mRNA decapping enzyme (AtDcp2).
Article in Nucleic acids research, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 32 citations in OpenAlex.
- mRNA decapping proteins regulate EIN2-dependent ethylene signaling in arabidopsis.Frontiers in plant science · 2026Article
- METTL3 promotes chemoresistance in small cell lung cancer by inducing mitophagy.Journal of experimental & clinical cancer research : CR · 2023Article
- The Mimivirus L375 Nudix enzyme hydrolyzes the 5' mRNA cap.PloS one · 2021Article
- Review
- Functional and molecular characterization of the conserved Arabidopsis PUMILIO protein, APUM9.Plant molecular biology · 2019Article
- Interconnections between mRNA degradation and RDR-dependent siRNA production in mRNA turnover in plants.Journal of plant research · 2017Review
- RNA Quality Control as a Key to Suppressing RNA Silencing of Endogenous Genes in Plants.Molecular plant · 2016Review
- Identification and characterization of Arabidopsis AtNUDX9 as a GDP-d-mannose pyrophosphohydrolase: its involvement in root growth inhibition in response to ammonium.Journal of experimental botany · 2015Article
- Structural Basis for the Specificity of Human NUDT16 and Its Regulation by Inosine Monophosphate.PloS one · 2015Article
- Insights into the molecular determinants involved in cap recognition by the vaccinia virus D10 decapping enzyme.Nucleic acids research · 2010Article
- Article
- Distinctive interactions of the Arabidopsis homolog of the 30 kD subunit of the cleavage and polyadenylation specificity factor (AtCPSF30) with other polyadenylation factor subunits.BMC cell biology · 2009Article
- A comprehensive bioinformatics analysis of the Nudix superfamily in Arabidopsis thaliana.Comparative and functional genomics · 2009Article
- Molecular characterization of organelle-type Nudix hydrolases in Arabidopsis.Plant physiology · 2008Article
- Transcriptome-wide analysis of uncapped mRNAs in Arabidopsis reveals regulation of mRNA degradation.The Plant cell · 2008Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Arabidopsis thaliana decapping enzyme (AtDcp2) was characterized by bioinformatics analysis and by biochemical studies of the enzyme and mutants produced by recombinant expression. Three functionally significant regions were detected: (i) a highly disordered C-terminal region with a putative PSD-95, Discs-large, ZO-1 (PDZ) domain-binding motif, (ii) a conserved Nudix box constituting the putative active site and (iii) a putative RNA binding domain consisting of the conserved Box B and a preceding loop region. Mutation of the putative PDZ domain-binding motif improved the stability of recombinant AtDcp2 and secondary mutants expressed in Escherichia coli. Such recombinant AtDcp2 specifically hydrolysed capped mRNA to produce 7-methyl GDP and decapped RNA. AtDcp2 activity was Mn(2+)- or Mg(2+)-dependent and was inhibited by the product 7-methyl GDP. Mutation of the conserved glutamate-154 and glutamate-158 in the Nudix box reduced AtDcp2 activity up to 400-fold and showed that AtDcp2 employs the catalytic mechanism conserved amongst Nudix hydrolases. Unlike many Nudix hydrolases, AtDcp2 is refractory to inhibition by fluoride ions. Decapping was dependent on binding to the mRNA moiety rather than to the 7-methyl diguanosine triphosphate cap of the substrate. Mutational analysis of the putative RNA-binding domain confirmed the functional significance of an 11-residue loop region and the conserved Box B.
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