ArticleThe Plant cell2007
Nitrate signaling by the regulatory gene NIT2 in Chlamydomonas.
Article in The Plant cell, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
54 citing papers in PubMed, 135 citations in OpenAlex.
- QuinoaFrontiers in plant science · 2026Article
- Genome-wide identification of the apple NLP (NIN-like proteins) family and their potential role under abiotic stress.3 Biotech · 2025Article
- Functional analysis of regA paralog rlsD in Volvox carteri.The Plant journal : for cell and molecular biology · 2024Article
- Genome-Wide Survey of theInternational journal of molecular sciences · 2023Article
- Identification and expression characteristics of NLP (NIN-like protein) gene family in pepper (Capsicum annuum L.).Molecular biology reports · 2023Article
- From Feasting to Fasting: The Arginine Pathway as a Metabolic Switch in Nitrogen-DeprivedCells · 2023Review
- Nitric oxide signaling in ctenophores.Frontiers in neuroscience · 2023Article
- Transcriptional dynamics of gametogenesis in the green seaweed Ulva mutabilis identifies an RWP-RK transcription factor linked to reproduction.BMC plant biology · 2022Article
- Genome sequencing of the multicellular alga Astrephomene provides insights into convergent evolution of germ-soma differentiation.Scientific reports · 2021Article
- Article
- Rice NIN-LIKE PROTEIN 4 plays a pivotal role in nitrogen use efficiency.Plant biotechnology journal · 2021Article
- The Loss of Function of theFrontiers in plant science · 2021Article
- The diversification and lineage-specific expansion of nitric oxide signaling in Placozoa: insights in the evolution of gaseous transmission.Scientific reports · 2020Article
- Nitrate in 2020: Thirty Years from Transport to Signaling Networks.The Plant cell · 2020Review
- Genome wide analysis of NLP transcription factors reveals their role in nitrogen stress tolerance of rice.Scientific reports · 2020Article
- Identification of the MAPK Cascade and its Relationship with Nitrogen Metabolism in the Green AlgaInternational journal of molecular sciences · 2020Article
- Evolutionary analyses of NIN-like proteins in plants and their roles in nitrate signaling.Cellular and molecular life sciences : CMLS · 2019Review
- Role of Nitrate Reductase in NO Production in Photosynthetic Eukaryotes.Plants (Basel, Switzerland) · 2019Review
- Molecular Regulation of Nitrate Responses in Plants.International journal of molecular sciences · 2018Review
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
Abstract
Positive signaling by nitrate in its assimilation pathway has been studied in Chlamydomonas reinhardtii. Among >34,000 lines generated by plasmid insertion, 10 mutants were unable to activate nitrate reductase (NIA1) gene expression and had a Nit(-) (no growth in nitrate) phenotype. Each of these 10 lines was mutated in the nitrate assimilation-specific regulatory gene NIT2. The complete NIT2 cDNA sequence was obtained, and its deduced amino acid sequence revealed GAF, Gln-rich, Leu zipper, and RWP-RK domains typical of transcription factors and transcriptional coactivators associated with signaling pathways. The predicted Nit2 protein sequence is structurally related to the Nin (for nodule inception) proteins from plants but not to NirA/Nit4/Yna proteins from fungi and yeast. NIT2 expression is negatively regulated by ammonium and is optimal in N-free medium with no need for the presence of nitrate. However, intracellular nitrate is required to allow Nit2 to activate the NIA1 promoter activity. Nit2 protein was expressed in Escherichia coli and shown to bind to specific sequences at the NIA1 gene promoter. Our data indicate that NIT2 is a central regulatory gene required for nitrate signaling on the Chlamydomonas NIA1 gene promoter and that intracellular nitrate is needed for NIT2 function and to modulate NIA1 transcript levels.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.