ArticleThe Plant cell2007
Downregulation of cinnamoyl-coenzyme A reductase in poplar: multiple-level phenotyping reveals effects on cell wall polymer metabolism and structure.
Article in The Plant cell, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 124 papers.
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Who cites it
124 citing papers in PubMed, 406 citations in OpenAlex.
- Integrative molecular mechanisms of lodging resistance in rice (Frontiers in plant science · 2026Review
- Integrated analysis of transcriptome and metabolome reveals the mechanism of lignin biosynthesis in fruit abscission of Chinese bayberry (Myrica rubra).BMC plant biology · 2025Article
- Integrative analyses reveal Bna-miR397a-BnaLAC2 as a potential modulator of low-temperature adaptability in Brassica napus L.Plant biotechnology journal · 2025Article
- Genome-wide identification and expression pattern analysis of the cinnamoyl-CoA reductase gene family in flax (Linum usitatissimum L.).BMC genomics · 2025Article
- From fibers to flowering to metabolites: unlocking hemp (Cannabis sativa) potential with the guidance of novel discoveries and tools.Journal of experimental botany · 2025Review
- Cinnamaldehyde in Focus: Antimicrobial Properties, Biosynthetic Pathway, and Industrial Applications.Antibiotics (Basel, Switzerland) · 2024Review
- Function Analysis of a Maize Endo-1,4-β-xylanase GeneInternational journal of molecular sciences · 2024Article
- Identification of a cinnamoyl-CoA reductase from Cinnamomum cassia involved in trans-cinnamaldehyde biosynthesis.Planta · 2024Article
- Silencing ScGUX2 reduces xylan glucuronidation and improves biomass saccharification in sugarcane.Plant biotechnology journal · 2024Article
- Article
- Suppression of the Arabidopsis cinnamoyl-CoA reductase 1-6 intronic T-DNA mutation by epigenetic modification.Plant physiology · 2023Article
- Pinoresinol rescues developmental phenotypes of Arabidopsis phenylpropanoid mutants overexpressingProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Metabolic engineering of p-hydroxybenzoate in poplar lignin.Plant biotechnology journal · 2023Article
- Microparticle-mediated CRISPR DNA delivery for genome editing in poplar.Frontiers in plant science · 2023Article
- Lignin engineering in forest trees: From gene discovery to field trials.Plant communications · 2022Review
- Flexible and digestible wood caused by viral-induced alteration of cell wall composition.Current biology : CB · 2022Article
- The chromosome-level genome foriScience · 2022Article
- A new approach to zip-lignin: 3,4-dihydroxybenzoate is compatible with lignification.The New phytologist · 2022Article
- Chloroplast Thylakoidal Ascorbate Peroxidase, PtotAPX, Has Enhanced Resistance to Oxidative Stress inInternational journal of molecular sciences · 2022Article
- Engineering lignin: getting more from woody biomass.Plant physiology · 2022Article
64 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
26 authors at 12 institutions in 6 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cinnamoyl-CoA reductase (CCR) catalyzes the penultimate step in monolignol biosynthesis. We show that downregulation of CCR in transgenic poplar (Populus tremula x Populus alba) was associated with up to 50% reduced lignin content and an orange-brown, often patchy, coloration of the outer xylem. Thioacidolysis, nuclear magnetic resonance (NMR), immunocytochemistry of lignin epitopes, and oligolignol profiling indicated that lignin was relatively more reduced in syringyl than in guaiacyl units. The cohesion of the walls was affected, particularly at sites that are generally richer in syringyl units in wild-type poplar. Ferulic acid was incorporated into the lignin via ether bonds, as evidenced independently by thioacidolysis and by NMR. A synthetic lignin incorporating ferulic acid had a red-brown coloration, suggesting that the xylem coloration was due to the presence of ferulic acid during lignification. Elevated ferulic acid levels were also observed in the form of esters. Transcript and metabolite profiling were used as comprehensive phenotyping tools to investigate how CCR downregulation impacted metabolism and the biosynthesis of other cell wall polymers. Both methods suggested reduced biosynthesis and increased breakdown or remodeling of noncellulosic cell wall polymers, which was further supported by Fourier transform infrared spectroscopy and wet chemistry analysis. The reduced levels of lignin and hemicellulose were associated with an increased proportion of cellulose. Furthermore, the transcript and metabolite profiling data pointed toward a stress response induced by the altered cell wall structure. Finally, chemical pulping of wood derived from 5-year-old, field-grown transgenic lines revealed improved pulping characteristics, but growth was affected in all transgenic lines tested.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.