ArticlePlant physiology2008
ECA3, a Golgi-localized P2A-type ATPase, plays a crucial role in manganese nutrition in Arabidopsis.
Article in Plant physiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 75 papers.
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Who cites it
75 citing papers in PubMed, 177 citations in OpenAlex.
- Advances in Imaging of Plant CaBiomolecules · 2026Review
- Genome-Wide Identification of the CaPlants (Basel, Switzerland) · 2026Article
- Calcium Signaling as an Emerging Integrator of Manganese Homeostasis inPlants (Basel, Switzerland) · 2026Review
- Resources of manganese hyperaccumulators and mechanisms of plant tolerance to excess manganese: A review.Planta · 2026Review
- Manganese handling in plants: Advances in the mechanistic and functional understanding of transport pathways.Quantitative plant biology · 2025Review
- Manganese: From Soil to Human Health-A Comprehensive Overview of Its Biological and Environmental Significance.Nutrients · 2024Review
- Review
- Golgi apparatus-localized CATION CALCIUM EXCHANGER4 promotes osmotolerance of Arabidopsis.Plant physiology · 2024Article
- AnPlant signaling & behavior · 2023Article
- Transcriptome Sequencing Analysis of Root in Soybean Responding to Mn Poisoning.International journal of molecular sciences · 2023Article
- Dissecting the relative contribution of ECA3 and group 8/9 cation diffusion facilitators to manganese homeostasis inPlant direct · 2023Article
- Review
- Comparative Transcriptome Analysis Reveals Complex Physiological Response and Gene Regulation in Peanut Roots and Leaves under Manganese Toxicity Stress.International journal of molecular sciences · 2023Article
- Manganese management in plants: Golgi transporter determines manganese allocation and cell wall composition.Plant physiology · 2022Article
- The trans-Golgi-localized protein BICAT3 regulates manganese allocation and matrix polysaccharide biosynthesis.Plant physiology · 2022Article
- Structure, Function, and Applications of Soybean Calcium Transporters.International journal of molecular sciences · 2022Review
- Genome-Wide Identification and Characterization of Banana CaInternational journal of molecular sciences · 2022Article
- CaProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Cellular-level distribution of manganese in Macadamia integrifolia, M. ternifolia, and M. tetraphylla from Australia.Metallomics : integrated biometal science · 2022Article
- The genome and preliminary single-nuclei transcriptome of Lemna minuta reveals mechanisms of invasiveness.Plant physiology · 2022Article
15 more citing papers are in PubMed but not listed here.
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8 authors at 4 institutions in 2 countries.
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Abstract
Calcium (Ca) and manganese (Mn) are essential nutrients required for normal plant growth and development, and transport processes play a key role in regulating their cellular levels. Arabidopsis (Arabidopsis thaliana) contains four P(2A)-type ATPase genes, AtECA1 to AtECA4, which are expressed in all major organs of Arabidopsis. To elucidate the physiological role of AtECA2 and AtECA3 in Arabidopsis, several independent T-DNA insertion mutant alleles were isolated. When grown on medium lacking Mn, eca3 mutants, but not eca2 mutants, displayed a striking difference from wild-type plants. After approximately 8 to 9 d on this medium, eca3 mutants became chlorotic, and root and shoot growth were strongly inhibited compared to wild-type plants. These severe deficiency symptoms were suppressed by low levels of Mn, indicating a crucial role for ECA3 in Mn nutrition in Arabidopsis. eca3 mutants were also more sensitive than wild-type plants and eca2 mutants on medium lacking Ca; however, the differences were not so striking because in this case all plants were severely affected. ECA3 partially restored the growth defect on high Mn of the yeast (Saccharomyces cerevisiae) pmr1 mutant, which is defective in a Golgi Ca/Mn pump (PMR1), and the yeast K616 mutant (Deltapmc1 Deltapmr1 Deltacnb1), defective in Golgi and vacuolar Ca/Mn pumps. ECA3 also rescued the growth defect of K616 on low Ca. Promoter:beta-glucuronidase studies show that ECA3 is expressed in a range of tissues and cells, including primary root tips, root vascular tissue, hydathodes, and guard cells. When transiently expressed in Nicotiana tabacum, an ECA3-yellow fluorescent protein fusion protein showed overlapping expression with the Golgi protein GONST1. We propose that ECA3 is important for Mn and Ca homeostasis, possibly functioning in the transport of these ions into the Golgi. ECA3 is the first P-type ATPase to be identified in plants that is required under Mn-deficient conditions.
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