ArticlePlant physiology2008
Functional analysis of the Kunitz trypsin inhibitor family in poplar reveals biochemical diversity and multiplicity in defense against herbivores.
Article in Plant physiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 126 citations in OpenAlex.
- The Circadian Clock Regulates Receptor-Mediated Immune Responses to a Herbivore-Associated Molecular Pattern.Plant, cell & environment · 2026Article
- Divergent functions of three Kunitz trypsin inhibitor (KTI) proteins in herbivore defense in poplar.BMC plant biology · 2025Article
- Multi-Transcripts and Expressions ofPlants (Basel, Switzerland) · 2025Article
- Kunitz-type trypsin inhibitor from durian (Durio zibethinus) employs a distinct loop for trypsin inhibition.Protein science : a publication of the Protein Society · 2024Article
- A Review of Bioactive Compound Effects from Primary Legume Protein Sources in Human and Animal Health.Current issues in molecular biology · 2024Review
- First Proteome Analysis of Poplar-Type Propolis.Plant foods for human nutrition (Dordrecht, Netherlands) · 2024Article
- Multi-omics analysis ofFrontiers in plant science · 2024Article
- Transcriptome Analysis Reveals Changes in Whole Gene Expression, Biological Process, and Molecular Functions Induced by Nickel in Jack Pine (Plants (Basel, Switzerland) · 2023Article
- Article
- Comparative analysis of herbivory responsive miRNAs to delineate pod borer (Helicoverpa armigera) resistance mechanisms in Cajanus cajan and its wild relative Cajanus scarabaeoides.Plant cell reports · 2022Article
- Poplar protease inhibitor expression differs in an herbivore specific manner.BMC plant biology · 2021Article
- Advances and Perspectives of Transgenic Technology and Biotechnological Application in Forest Trees.Frontiers in plant science · 2021Review
- Comparative Analysis Delineates the Transcriptional Resistance Mechanisms for Pod Borer Resistance in the Pigeonpea Wild RelativeInternational journal of molecular sciences · 2020Article
- Host plant genetic control of associated fungal and insect species in aEcology and evolution · 2020Article
- Genome-Wide Association Study of Wood Anatomical and Morphological Traits inFrontiers in plant science · 2020Article
- SEGN: Inferring real-time gene networks mediating phenotypic plasticity.Computational and structural biotechnology journal · 2020Article
- Model prediction of a Kunitz-type trypsin inhibitor protein from seeds of Acacia nilotica L. with strong antimicrobial and insecticidal activity.Turkish journal of biology = Turk biyoloji dergisi · 2020Article
- Rhizophagus intraradices promotes alfalfa (Medicago sativa) defense against pea aphids (Acyrthosiphon pisum) revealed by RNA-Seq analysis.Mycorrhiza · 2019Article
- Separate Pathways Contribute to the Herbivore-Induced Formation of 2-Phenylethanol in Poplar.Plant physiology · 2019Article
- Plant Serine Protease Inhibitors: Biotechnology Application in Agriculture and Molecular Farming.International journal of molecular sciences · 2019Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We investigated the functional and biochemical variability of Kunitz trypsin inhibitor (KTI) genes of Populus trichocarpa x Populus deltoides. Phylogenetic analysis, expressed sequence tag databases, and western-blot analysis confirmed that these genes belong to a large and diverse gene family with complex expression patterns. Five wound- and herbivore-induced genes representing the diversity of the KTI gene family were selected for functional analysis and shown to produce active KTI proteins in Escherichia coli. These recombinant KTI proteins were all biochemically distinct and showed clear differences in efficacy against trypsin-, chymotrypsin-, and elastase-type proteases, suggesting functional specialization of different members of this gene family. The in vitro stability of the KTIs in the presence of reducing agents and elevated temperature also varied widely, emphasizing the biochemical differences of these proteins. Significantly, the properties of the recombinant KTI proteins were not predictable from primary amino acid sequence data. Proteases in midgut extracts of Malacosoma disstria, a lepidopteran pest of Populus, were strongly inhibited by at least two of the KTI gene products. This study suggests that the large diversity in the poplar (Populus spp.) KTI family is important for biochemical and functional specialization, which may be important in the maintenance of pest resistance in long-lived plants such as poplar.
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