ArticleBiophysical journal2008
Spatial distribution of maxi-anion channel on cardiomyocytes detected by smart-patch technique.
Article in Biophysical journal, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 51 citations in OpenAlex.
- Effect of VSOR/VRAC blocker tamoxifen on contractile activity of primary neonatal cardiomyocytes and isolated perfused heart.BMC research notes · 2026Article
- Review
- Scanning Ion Conductance Microscopy.Chemical reviews · 2021Review
- The ATP-Releasing Maxi-Cl Channel: Its Identity, Molecular Partners and Physiological/Pathophysiological Implications.Life (Basel, Switzerland) · 2021Review
- Cell Death Induction and Protection by Activation of Ubiquitously Expressed Anion/Cation Channels. Part 1: Roles of VSOR/VRAC in Cell Volume Regulation, Release of Double-Edged Signals and Apoptotic/Necrotic Cell Death.Frontiers in cell and developmental biology · 2020Review
- The organic anion transporter SLCO2A1 constitutes the core component of the Maxi-Cl channel.The EMBO journal · 2017Article
- Cell culture: complications due to mechanical release of ATP and activation of purinoceptors.Cell and tissue research · 2017Review
- Angular Approach Scanning Ion Conductance Microscopy.Biophysical journal · 2016Article
- The properties, functions, and pathophysiology of maxi-anion channels.Pflugers Archiv : European journal of physiology · 2016Review
- Cardiac purinergic signalling in health and disease.Purinergic signalling · 2015Review
- Super-resolution scanning patch clamp reveals clustering of functional ion channels in adult ventricular myocyte.Circulation research · 2013Article
- Volume-sensitive anion channels mediate osmosensitive glutathione release from rat thymocytes.PloS one · 2013Article
- Scanning ion conductance microscopy for studying biological samples.Sensors (Basel, Switzerland) · 2012Review
- Scanning ion conductance microscopy: a nanotechnology for biological studies in live cells.Frontiers in physiology · 2012Article
- Swelling-activated anion channels are essential for volume regulation of mouse thymocytes.International journal of molecular sciences · 2011Article
- Measurement of the thickness and volume of adherent cells using transmission-through-dye microscopy.Pflugers Archiv : European journal of physiology · 2010Article
- Purinergic signaling: a fundamental mechanism in neutrophil activation.Science signaling · 2010Article
- The maxi-anion channel: a classical channel playing novel roles through an unidentified molecular entity.The journal of physiological sciences : JPS · 2009Review
Corrections and comments
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Authors and funding
6 authors at 5 institutions in 3 countries.
Funding
Abstract
Spatial distribution of maxi-anion channels in rat cardiomyocytes were studied by applying the recently developed patch clamp technique under scanning ion conductance microscopy, called the "smart-patch" technique. In primary-cultured neonatal cells, the channel was found to be unevenly distributed over the cell surface with significantly lower channel activity in cellular extensions compared with the other parts. Local ATP release, detected using a PC12 cell-based biosensor technique, also exhibited a similar pattern. The maxi-anion channel activity could not be detected in freshly isolated adult cardiomyocytes by the conventional patch-clamp with 2-MOmega pipettes. However, when fine-tipped 15-20 MOmega pipettes were targeted to only Z-line areas, we observed, for the first time, the maxi-anion events. Smart-patching different regions of the cell surface, we found that the channel activity was maximal at the openings of T-tubules and along Z-lines, but was significantly decreased in the scallop crest area. Thus, it is concluded that maxi-anion channels are concentrated at the openings of T-tubules and along Z-lines in adult cardiomyocytes. This study showed that the smart-patch technique provides a powerful method to detect a unitary event of channels that are localized at some specific site in the narrow region.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.