ArticleCurrent biology : CB2007
A whole-genome RNAi Screen for C. elegans miRNA pathway genes.
Article in Current biology : CB, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
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Who cites it
60 citing papers in PubMed.
- Regulation of MAP Kinase signaling by the insulin-like growth factor pathway duringmicroPublication biology · 2025Article
- RNA-binding proteins and exoribonucleases modulating miRNA in cancer: the enemy within.Experimental & molecular medicine · 2024Review
- An RNAi screen for conserved kinases that enhance microRNA activity after dauer in Caenorhabditis elegans.G3 (Bethesda, Md.) · 2024Article
- Recent advances in understanding microRNA function and regulation in C. elegans.Seminars in cell & developmental biology · 2024Review
- Lipid kinase PIP5K1A regulates let-7 microRNA biogenesis through interacting with nuclear export protein XPO5.Nucleic acids research · 2023Article
- Identifying the Caenorhabditis elegans vulval transcriptome.G3 (Bethesda, Md.) · 2022Article
- Functional identification of microRNA-centered complexes in C. elegans.Scientific reports · 2022Article
- Article
- The RabGAP TBC-11 controls Argonaute localization for proper microRNA function in C. elegans.PLoS genetics · 2021Article
- KH domain containing RNA-binding proteins coordinate with microRNAs to regulate Caenorhabditis elegans development.G3 (Bethesda, Md.) · 2021Article
- Xrn1p acts at multiple steps in the budding-yeast RNAi pathway to enhance the efficiency of silencing.Nucleic acids research · 2020Article
- HRPK-1, a conserved KH-domain protein, modulates microRNA activity during Caenorhabditis elegans development.PLoS genetics · 2019Article
- Recent Molecular Genetic Explorations ofGenetics · 2018Article
- Article
- Tag team: Roles of miRNAs and Proteolytic Regulators in Ensuring Robust Gene Expression Dynamics.Trends in genetics : TIG · 2018Review
- A continuum of mRNP complexes in embryonic microRNA-mediated silencing.Nucleic acids research · 2017Article
- Article
- Asian Citrus Psyllid RNAi Pathway - RNAi evidence.Scientific reports · 2016Article
- A quantitative genome-wide RNAi screen in C. elegans for antifungal innate immunity genes.BMC biology · 2016Article
- SmD1 Modulates the miRNA Pathway Independently of Its Pre-mRNA Splicing Function.PLoS genetics · 2015Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
backgroundmiRNAs are an abundant class of small, endogenous regulatory RNAs. Although it is now appreciated that miRNAs are involved in a broad range of biological processes, relatively little is known about the actual mechanism by which miRNAs downregulate target gene expression. An exploration of which protein cofactors are necessary for a miRNA to downregulate a target gene should reveal more fully the molecular mechanisms by which miRNAs are processed, trafficked, and regulate their target genes.
resultsA weak allele of the C. elegans miRNA gene let-7 was used as a sensitized genetic background for a whole-genome RNAi screen to detect miRNA pathway genes, and 213 candidate miRNA pathway genes were identified. About 2/3 of the 61 candidates with the strongest phenotype were validated through genetic tests examining the dependence of the let-7 phenotype on target genes known to function in the let-7 pathway. Biochemical tests for let-7 miRNA production place the function of nearly all of these new miRNA pathway genes downstream of let-7 expression and processing. By monitoring the downregulation of the protein product of the lin-14 mRNA, which is the target of the lin-4 miRNA, we have identified 19 general miRNA pathway genes.
conclusionsThe 213 candidate miRNA pathway genes identified could act at steps that produce and traffic miRNAs or in downstream steps that detect miRNA::mRNA duplexes to regulate mRNA translation. The 19 validated general miRNA pathway genes are good candidates for genes that may define protein cofactors for sorting or targeting miRNA::mRNA duplexes, or for recognizing the miRNA base-paired to the target mRNA to downregulate translation.
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