ArticleMatrix biology : journal of the International Society for Matrix Biology2008
Expression of two novel alternatively spliced COL2A1 isoforms during chondrocyte differentiation.
Article in Matrix biology : journal of the International Society for Matrix Biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 34 citations in OpenAlex.
- A Single-cell Atlas of Developing Mouse Palates Reveals Cellular and Molecular Transitions in Periderm Cell Fate.Genomics, proteomics & bioinformatics · 2025Article
- Alternative Splicing: A Critical Regulator in Human Bone Biology and Tumor Progression.Research (Washington, D.C.) · 2025Review
- Presence of type IIB procollagen in mouse articular cartilage and growth plate is revealed by immuno-histochemical analysis with a novel specific antibody.Matrix biology plus · 2023Article
- A Novel missense mutation of COL2A1 gene in a large family with stickler syndrome type I.Journal of cellular and molecular medicine · 2022Article
- The pattern of duck sternal ossification and the changes of histological structure and gene expression therein.Poultry science · 2021Article
- Long-term expansion, enhanced chondrogenic potential, and suppression of endochondral ossification of adult human MSCs via WNT signaling modulation.Stem cell reports · 2015Article
- An evaluation of the delayed effect of intra-articular injections of lidocaine (2%) on articular cartilage: an experimental study in rabbits.European journal of orthopaedic surgery & traumatology : orthopedie traumatologie · 2014Article
- Alternative splicing of type II procollagen: IIB or not IIB?Connective tissue research · 2014Review
- Changes in type II procollagen isoform expression during chondrogenesis by disruption of an alternative 5' splice site within Col2a1 exon 2.Matrix biology : journal of the International Society for Matrix Biology · 2014Article
- Characterization of a murine type IIB procollagen-specific antibody.Matrix biology : journal of the International Society for Matrix Biology · 2014Article
- Molecular properties and fibril ultrastructure of types II and XI collagens in cartilage of mice expressing exclusively the α1(IIA) collagen isoform.Matrix biology : journal of the International Society for Matrix Biology · 2014Article
- Disruption of the developmentally-regulated Col2a1 pre-mRNA alternative splicing switch in a transgenic knock-in mouse model.Matrix biology : journal of the International Society for Matrix Biology · 2012Article
- Changes of chondrocyte expression profiles in human MSC aggregates in the presence of PEG microspheres and TGF-β3.Biomaterials · 2011Article
- Hydrolyzed fish collagen induced chondrogenic differentiation of equine adipose tissue-derived stromal cells.Histochemistry and cell biology · 2010Article
- BMP-2 and TGF-beta1 differentially control expression of type II procollagen and alpha 10 and alpha 11 integrins in mouse chondrocytes.European journal of cell biology · 2010Article
- Quantification of type II procollagen splice forms using alternative transcript-qPCR (AT-qPCR).Matrix biology : journal of the International Society for Matrix BiologyArticle
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Alternative splicing of the type II procollagen gene (COL2A1) is developmentally regulated during chondrogenesis. Type IIA procollagen (+ exon 2) is synthesized by chondroprogenitor cells while type IIB procollagen (- exon 2) is synthesized by differentiated chondrocytes. Here, we report expression of two additional alternatively spliced COL2A1 isoforms during chondrocyte differentiation of bone marrow-derived mesenchymal stem cells (MSCs). One isoform, named IIC, contains only the first 34 nucleotides of exon 2 by the use of an alternative 5' splice site, resulting in a premature termination codon and possible nonsense-mediated decay of IIC mRNA. Low levels of the IIC isoform were detected by RT-PCR and Southern analysis of COL2A1 cDNA amplified from differentiating rabbit and human MSCs. A second novel transcript, named IID, arises by the use of another 5' alternative splice site in intron 2. The IID isoform contains exon 2 plus 3 nucleotides, resulting in the insertion of an additional amino acid. The IID isoform was co-expressed with the IIA isoform during chondrogenesis, and was approximately one-third as abundant. Deletion of the IIC alternative 5' splice site from a COL2A1 mini-gene construct resulted in a significant increase in the IIA:IIB ratio. A mutant mini-gene that inhibited production of the IID isoform, however, had differential effects on the production of the IIA and IIB isoforms: this was apparently related to the differentiation status of the cell type used. These data suggest that COL2A1 mRNA abundance and other aspects of chondrocyte differentiation may be regulated by the use of these previously undetermined alternative splice sites.
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