ArticleExperimental parasitology2008
Trypanosoma brucei: differential requirement of membrane potential for import of proteins into mitochondria in two developmental stages.
Article in Experimental parasitology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 23 citations in OpenAlex.
- Thermo-related degeneration of stumpy forms of Trypanosoma brucei, the pathogen of African sleeping sickness.Advanced biotechnology · 2025Article
- Leishmania major Dihydrolipoyl dehydrogenase (DLD) is a key metabolic enzyme that drives parasite proliferation, pathology and host immune response.PLoS pathogens · 2025Article
- Multi-target mode of action of a Clerodane-type diterpenoid from Polyalthia longifolia targeting African trypanosomes.Scientific reports · 2018Article
- In vivo effect of Commiphora swynnertonii ethanolic extracts on Trypanosoma congolense and selected immunological components in mice.BMC complementary and alternative medicine · 2017Article
- Antitrypanosomal activity of Verbascum sinaiticum Benth. (Scrophulariaceae) against Trypanosoma congolense isolates.BMC complementary and alternative medicine · 2016Article
- Trypanocidal action of bisphosphonium salts through a mitochondrial target in bloodstream form Trypanosoma brucei.International journal for parasitology. Drugs and drug resistance · 2016Article
- Tim62, a Novel Mitochondrial Protein in Trypanosoma brucei, Is Essential for Assembly and Stability of the TbTim17 Protein Complex.The Journal of biological chemistry · 2015Article
- The krebs cycle enzyme α-ketoglutarate decarboxylase is an essential glycosomal protein in bloodstream African trypanosomes.Eukaryotic cell · 2015Article
- Trypanosome alternative oxidase possesses both an N-terminal and internal mitochondrial targeting signal.Eukaryotic cell · 2014Article
- Tim50 in Trypanosoma brucei possesses a dual specificity phosphatase activity and is critical for mitochondrial protein import.The Journal of biological chemistry · 2013Article
- Euglena gracilis and Trypanosomatids possess common patterns in predicted mitochondrial targeting presequences.Journal of molecular evolution · 2012Article
- Protein translocase of mitochondrial inner membrane in Trypanosoma brucei.The Journal of biological chemistry · 2012Article
- Article
- Role of Tob55 on mitochondrial protein biogenesis in Trypanosoma brucei.Molecular and biochemical parasitology · 2010Article
- Characterization of the mitochondrial inner membrane protein translocator Tim17 from Trypanosoma brucei.Molecular and biochemical parasitology · 2008Article
- The mitochondrion is a site of trypanocidal action of the aromatic diamidine DB75 in bloodstream forms of Trypanosoma brucei.Antimicrobial agents and chemotherapy · 2008Article
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Authors and funding
4 authors at 1 institution in 1 country.
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Abstract
Trypanosome alternative oxidase (TAO) and the cytochrome oxidase (COX) are two developmentally regulated terminal oxidases of the mitochondrial electron transport chain in Trypanosoma brucei. Here, we have compared the import of TAO and cytochrome oxidase subunit IV (COIV), two stage-specific nuclear encoded mitochondrial proteins, into the bloodstream and procyclic form mitochondria of T. brucei to understand the import processes in two different developmental stages. Under in vitro conditions TAO and COIV were imported and processed into isolated mitochondria from both the bloodstream and procyclic forms. With mitochondria isolated from the procyclic form, the import of TAO and COIV was dependent on the mitochondrial inner membrane potential (delta psi) and required protein(s) on the outer membrane. Import of these proteins also depended on the presence of both internal and external ATP. However, import of TAO and COIV into isolated mitochondria from the bloodstream form was not inhibited after the mitochondrial delta psi was dissipated by valinomycin, CCCP, or valinomycin and oligomycin in combination. In contrast, import of these proteins into bloodstream mitochondria was abolished after the hydrolysis of ATP by apyrase or removal of the ATP and ATP-generating system, suggesting that import is dependent on the presence of external ATP. Together, these data suggest that nuclear encoded proteins such as TAO and COIV are imported in the mitochondria of the bloodstream and the procyclic forms via different mechanism. Differential import conditions of nuclear encoded mitochondrial proteins of T. brucei possibly help it to adapt to different life forms.
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