ArticlePLoS genetics2007
The "alternative" choice of constitutive exons throughout evolution.
Article in PLoS genetics, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed.
- Augmenting transcriptome annotations through the lens of splicing evolution.Genome research · 2026Article
- The splice of life: how alternative splicing shapes regulatory and phenotypic evolution.The EMBO journal · 2026Review
- Exon Nomenclature And Classification of Transcripts (ENACT) provides a systematic framework to annotate exon attributes.Genome research · 2025Article
- The Role of Alternative Splicing in Marine-Freshwater Divergence in Threespine Stickleback.Genome biology and evolution · 2025Article
- Article
- Evolutionarily Developed Alternatively Spliced Exons Containing Translation Initiation Sites.Cells · 2024Article
- Article
- Splicing complexity as a pivotal feature of alternative exons in mammalian species.BMC genomics · 2023Article
- Branch point strength controls species-specificLife science alliance · 2023Article
- Alternative splicing during mammalian organ development.Nature genetics · 2021Article
- Sequence and Evolutionary Features for the Alternatively Spliced Exons of Eukaryotic Genes.International journal of molecular sciences · 2019Review
- Evolution of Alternative Splicing in Eudicots.Frontiers in plant science · 2019Article
- Human canonical CD157/Bst1 is an alternatively spliced isoform masking a previously unidentified primate-specific exon included in a novel transcript.Scientific reports · 2017Article
- Identification of protein features encoded by alternative exons using Exon Ontology.Genome research · 2017Article
- Does conservation account for splicing patterns?BMC genomics · 2016Article
- Proteomic Analysis of Parkin Isoforms Expression in Different Rat Brain Areas.The protein journal · 2016Article
- Selection preserves Ubiquitin Specific Protease 4 alternative exon skipping in therian mammals.Scientific reports · 2016Article
- Diverse regulation of 3' splice site usage.Cellular and molecular life sciences : CMLS · 2015Review
- The role of Alu elements in the cis-regulation of RNA processing.Cellular and molecular life sciences : CMLS · 2015Review
- Increasing the Coding Potential of Genomes Through Alternative Splicing: The Case of PARK2 Gene.Current genomics · 2014Article
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Alternative cassette exons are known to originate from two processes-exonization of intronic sequences and exon shuffling. Herein, we suggest an additional mechanism by which constitutively spliced exons become alternative cassette exons during evolution. We compiled a dataset of orthologous exons from human and mouse that are constitutively spliced in one species but alternatively spliced in the other. Examination of these exons suggests that the common ancestors were constitutively spliced. We show that relaxation of the 5' splice site during evolution is one of the molecular mechanisms by which exons shift from constitutive to alternative splicing. This shift is associated with the fixation of exonic splicing regulatory sequences (ESRs) that are essential for exon definition and control the inclusion level only after the transition to alternative splicing. The effect of each ESR on splicing and the combinatorial effects between two ESRs are conserved from fish to human. Our results uncover an evolutionary pathway that increases transcriptome diversity by shifting exons from constitutive to alternative splicing.
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