ArticleJournal of molecular neuroscience : MN2008
Gephyrin selective intrabodies as a new strategy for studying inhibitory receptor clustering.
Article in Journal of molecular neuroscience : MN, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 15 citations in OpenAlex.
- Genetically encoded intrabodies as high-precision tools to visualize and manipulate neuronal function.Seminars in cell & developmental biology · 2022Article
- Recombinant Antibodies in Basic Neuroscience Research.Current protocols in neuroscience · 2020Review
- Tuning GABAergic Inhibition: Gephyrin Molecular Organization and Functions.Neuroscience · 2020Review
- Computational Modeling of Inhibitory Transsynaptic Signaling in Hippocampal and Cortical Neurons Expressing Intrabodies Against Gephyrin.Frontiers in cellular neuroscience · 2020Article
- Targeting GABAFrontiers in molecular neuroscience · 2019Article
- Impaired Glycine Receptor Trafficking in Neurological Diseases.Frontiers in molecular neuroscience · 2018Review
- A General Procedure to Study Subcellular Models of Transsynaptic Signaling at Inhibitory Synapses.Frontiers in neuroinformatics · 2016Article
- Review
- Gephyrin regulates GABAergic and glutamatergic synaptic transmission in hippocampal cell cultures.The Journal of biological chemistry · 2011Article
- Cellular transport and membrane dynamics of the glycine receptor.Frontiers in molecular neuroscience · 2009Article
- Sequences Flanking the Gephyrin-Binding Site of GlyRβ Tune Receptor Stabilization at Synapses.eNeuroArticle
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The microtubule-binding protein gephyrin is known to play a pivotal role in targeting and clustering postsynaptic inhibitory receptors. Here, the Intracellular Antibodies Capture Technology (IATC) was used to select two single-chain antibody fragments or intrabodies, which, fused to nuclear localization signals (NLS), were able to efficiently and selectively remove gephyrin from glycine receptor (GlyR) clusters. Co-transfection of NLS-tagged individual intrabodies with gephyrin-enhanced green fluorescent protein (EGFP) in HEK 293 cells revealed a partial relocalization of gephyrin aggregates onto the nucleus or in the perinuclear area. When expressed in cultured neurons, these intrabodies caused a significant reduction in the number of immunoreactive GlyR clusters, which was associated with a decrease in the peak amplitude of glycine-evoked whole cell currents as assessed with electrophysiological experiments. Hampering protein function at a posttranslational level may represent an attractive alternative for interfering with gephyrin function in a more spatially localized manner.
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Registered trials
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