ArticleNature protocols2007
Construction of conditional analog-sensitive kinase alleles in the fission yeast Schizosaccharomyces pombe.
Article in Nature protocols, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 50 citations in OpenAlex.
- The DNA Damage Repair Function of Fission Yeast CK1 Involves Targeting Arp8, a Subunit of the INO80 Chromatin Remodeling Complex.Molecular and cellular biology · 2024Article
- A phosphoinositide hub connects CLE peptide signaling and polar auxin efflux regulation.Nature communications · 2023Article
- Kinase domain autophosphorylation rewires the activity and substrate specificity of CK1 enzymes.Molecular cell · 2022Article
- Phosphorylation in the intrinsically disordered region of F-BAR protein Imp2 regulates its contractile ring recruitment.Journal of cell science · 2021Article
- Substrate Phosphorylation Rates as an In Vivo Measurement of Kinase Activity.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Phosphoproteomics Meets Chemical Genetics: Approaches for Global Mapping and Deciphering the Phosphoproteome.International journal of molecular sciences · 2020Review
- Phosphoregulation of the cytokinetic protein Fic1 contributes to fission yeast growth polarity establishment.Journal of cell science · 2020Article
- Mutations that prevent methylation of cohesin render sensitivity to DNA damage inJournal of cell science · 2018Article
- RNA metabolism is the primary target of formamide in vivo.Scientific reports · 2017Article
- Dialogue between centrosomal entrance and exit scaffold pathways regulates mitotic commitment.The Journal of cell biology · 2017Article
- A drug-compatible and temperature-controlled microfluidic device for live-cell imaging.Open biology · 2016Article
- Dbl2 Regulates Rad51 and DNA Joint Molecule Metabolism to Ensure Proper Meiotic Chromosome Segregation.PLoS genetics · 2016Article
- Prp4 Kinase Grants the License to Splice: Control of Weak Splice Sites during Spliceosome Activation.PLoS genetics · 2016Article
- Allele-sensitive mutant, Itkas, reveals that Itk kinase activity is required for Th1, Th2, Th17, and iNKT-cell cytokine production.European journal of immunology · 2015Article
- Casein Kinase 1 and Phosphorylation of Cohesin Subunit Rec11 (SA3) Promote Meiotic Recombination through Linear Element Formation.PLoS genetics · 2015Article
- Optimization of the analogue-sensitive Cdc2/Cdk1 mutant by in vivo selection eliminates physiological limitations to its use in cell cycle analysis.Open biology · 2014Article
- DNA intermediates of meiotic recombination in synchronous S. pombe at optimal temperature.Nucleic acids research · 2014Article
- Synchronized fission yeast meiosis using an ATP analog-sensitive Pat1 protein kinase.Nature protocols · 2014Article
- A new tool for an old problem: synchronizing fission yeast cells during meiosis using an ATP analog-sensitive protein kinase.Cell cycle (Georgetown, Tex.) · 2012Article
- ATP analog-sensitive Pat1 protein kinase for synchronous fission yeast meiosis at physiological temperature.Cell cycle (Georgetown, Tex.) · 2012Article
Corrections and comments
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Authors and funding
8 authors at 6 institutions in 3 countries.
Funding
Abstract
Reversible protein phosphorylation is a major regulatory mechanism in a cell. A chemical-genetic strategy to conditionally inactivate protein kinases has been developed recently. Mutating a single residue in the ATP-binding pocket confers sensitivity to small-molecule inhibitors. The inhibitor can only bind to the mutant kinase and not to any other wild-type kinase, allowing specific inactivation of the modified kinase. Here, we describe a protocol to construct conditional analog-sensitive kinase alleles in the fission yeast Schizosaccharomyces pombe. This protocol can be completed in about 3 weeks and should be applicable to other organisms as well.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.