ArticleThe EMBO journal2007
Sec18p and Vam7p remodel trans-SNARE complexes to permit a lipid-anchored R-SNARE to support yeast vacuole fusion.
Article in The EMBO journal, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 37 citations in OpenAlex.
- Energetics, kinetics, and pathways of SNARE assembly in membrane fusion.Critical reviews in biochemistry and molecular biology · 2022Review
- The Transmembrane Domain of Synaptobrevin Influences Neurotransmitter Flux through Synaptic Fusion Pores.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2018Article
- A short region upstream of the yeast vacuolar Qa-SNARE heptad-repeats promotes membrane fusion through enhanced SNARE complex assembly.Molecular biology of the cell · 2017Article
- ESCRTs function directly on the lysosome membrane to downregulate ubiquitinated lysosomal membrane proteins.eLife · 2017Article
- A cascade of multiple proteins and lipids catalyzes membrane fusion.Molecular biology of the cell · 2017Review
- Vacuolar SNARE protein transmembrane domains serve as nonspecific membrane anchors with unequal roles in lipid mixing.The Journal of biological chemistry · 2015Article
- Sec17 can trigger fusion of trans-SNARE paired membranes without Sec18.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Yeast vacuolar HOPS, regulated by its kinase, exploits affinities for acidic lipids and Rab:GTP for membrane binding and to catalyze tethering and fusion.Molecular biology of the cell · 2015Article
- Impairment of autophagosome-lysosome fusion in the buff mutant mice with the VPS33A(D251E) mutation.Autophagy · 2015Article
- The Gos28 SNARE protein mediates intra-Golgi transport of rhodopsin and is required for photoreceptor survival.The Journal of biological chemistry · 2014Article
- Differential gradients of interaction affinities drive efficient targeting and recycling in the GET pathway.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Article
- Lipid-anchored SNAREs lacking transmembrane regions fully support membrane fusion during neurotransmitter release.Neuron · 2013Article
- The lipid composition and physical properties of the yeast vacuole affect the hemifusion-fusion transition.Traffic (Copenhagen, Denmark) · 2013Article
- Importance of the N-terminal domain of the Qb-SNARE Vti1p for different membrane transport steps in the yeast endosomal system.PloS one · 2013Article
- A lipid-anchored SNARE supports membrane fusion.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Phosphoinositides function asymmetrically for membrane fusion, promoting tethering and 3Q-SNARE subcomplex assembly.The Journal of biological chemistry · 2010Article
- HOPS prevents the disassembly of trans-SNARE complexes by Sec17p/Sec18p during membrane fusion.The EMBO journal · 2010Article
- Transmembrane protein-free membranes fuse into xenopus nuclear envelope and promote assembly of functional pores.The Journal of biological chemistry · 2009Article
- Phosphoinositides and SNARE chaperones synergistically assemble and remodel SNARE complexes for membrane fusion.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Intracellular membrane fusion requires SNARE proteins in a trans-complex, anchored to apposed membranes. Proteoliposome studies have suggested that SNAREs drive fusion by stressing the lipid bilayer via their transmembrane domains (TMDs), and that SNARE complexes require a TMD in each docked membrane to promote fusion. Yeast vacuole fusion is believed to require three Q-SNAREs from one vacuole and the R-SNARE Nyv1p from its fusion partner. In accord with this model, we find that fusion is abolished when the TMD of Nyv1p is replaced by lipid anchors, even though lipid-anchored Nyv1p assembles into trans-SNARE complexes. However, normal fusion is restored by the addition of both Sec18p and the soluble SNARE Vam7p. In restoring fusion, Sec18p promotes the disassembly of trans-SNARE complexes, and Vam7p enhances their assembly. Thus, either the TMD of this R-SNARE is not essential for fusion, and TMD-mediated membrane stress is not the only mode of trans-SNARE complex action, or these SNAREs have more flexibility than heretofore appreciated to form alternate functional complexes that violate the 3Q:1R rule.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.