ArticleThe EMBO journal2007
The CENP-A NAC/CAD kinetochore complex controls chromosome congression and spindle bipolarity.
Article in The EMBO journal, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
60 citing papers in PubMed, 81 citations in OpenAlex.
- FOXM1 Signaling Network Transcriptionally Upregulates Expression of Proteins Involved in Mitotic Progression to Induce High Proliferation and Chromosomal Instability in Androgen Receptor-Low Triple-Negative Breast Cancer.International journal of molecular sciences · 2026Article
- Independence of centromeric and pericentromeric chromatin stability on CCAN components.Molecular biology of the cell · 2025Article
- β-TrCP-Mediated Proteolysis of Mis18β Prevents Mislocalization of CENP-A and Chromosomal Instability.Molecular and cellular biology · 2024Article
- The Four Causes: The Functional Architecture of Centromeres and Kinetochores.Annual review of genetics · 2022Review
- Augmin-dependent microtubule self-organization drives kinetochore fiber maturation in mammals.Cell reports · 2022Article
- Kinetochore assembly throughout the cell cycle.Seminars in cell & developmental biology · 2021Article
- CENPN Acts as a Novel Biomarker that Correlates With the Malignant Phenotypes of Glioma Cells.Frontiers in genetics · 2021Article
- Structure of the Human Core Centromeric Nucleosome Complex.Current biology : CB · 2019Article
- Reconstitution of a 26-Subunit Human Kinetochore Reveals Cooperative Microtubule Binding by CENP-OPQUR and NDC80.Molecular cell · 2018Article
- p37/UBXN2B regulates spindle orientation by limiting cortical NuMA recruitment via PP1/Repo-Man.The Journal of cell biology · 2018Article
- Article
- Overexpression of the E2F target geneOncotarget · 2017Article
- Mechanisms of Chromosome Congression during Mitosis.Biology · 2017Review
- A Molecular View of Kinetochore Assembly and Function.Biology · 2017Review
- NuSAP governs chromosome oscillation by facilitating the Kid-generated polar ejection force.Nature communications · 2016Article
- The CENP-L-N Complex Forms a Critical Node in an Integrated Meshwork of Interactions at the Centromere-Kinetochore Interface.Molecular cell · 2015Article
- LSD1 is essential for oocyte meiotic progression by regulating CDC25B expression in mice.Nature communications · 2015Article
- Article
- CENP-C is a blueprint for constitutive centromere-associated network assembly within human kinetochores.The Journal of cell biology · 2015Article
- Structural transitions of centromeric chromatin regulate the cell cycle-dependent recruitment of CENP-N.Genes & development · 2015Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Kinetochores are complex protein machines that link chromosomes to spindle microtubules and contain a structural core composed of two conserved protein-protein interaction networks: the well-characterized KMN (KNL1/MIND/NDC80) and the recently identified CENP-A NAC/CAD. Here we show that the CENP-A NAC/CAD subunits can be assigned to one of two different functional classes; depletion of Class I proteins (Mcm21R(CENP-O) and Fta1R(CENP-L)) causes a failure in bipolar spindle assembly. In contrast, depletion of Class II proteins (CENP-H, Chl4R(CENP-N), CENP-I and Sim4R(CENP-K)) prevents binding of Class I proteins and causes chromosome congression defects, but does not perturb spindle formation. Co-depletion of Class I and Class II proteins restores spindle bipolarity, suggesting that Class I proteins regulate or counteract the function of Class II proteins. We also demonstrate that CENP-A NAC/CAD and KMN regulate kinetochore-microtubule attachments independently, even though CENP-A NAC/CAD can modulate NDC80 levels at kinetochores. Based on our results, we propose that the cooperative action of CENP-A NAC/CAD subunits and the KMN network drives efficient chromosome segregation and bipolar spindle assembly during mitosis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.