ArticleThe Journal of physiology2008
Reactive oxygen species and fatigue-induced prolonged low-frequency force depression in skeletal muscle fibres of rats, mice and SOD2 overexpressing mice.
Article in The Journal of physiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers, 1 of them a synthesis that pooled it.
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Who cites it
63 citing papers in PubMed, 1 synthesis or guideline pooled it, 139 citations in OpenAlex.
- Mechanisms underlying performance impairments following prolonged static stretching without a comprehensive warm-up.European journal of applied physiology · 2021Pooled it
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- Ryanodine receptor fragmentation and sarcoplasmic reticulum Ca2+ leak after one session of high-intensity interval exercise.Proceedings of the National Academy of Sciences of the United States of America · 2015Trial
- Mitochondrial bioenergetics in resilience of older adults with gynecologic cancer: design and rationale of a pilot study.GeroScience · 2026Article
- Competing effects of activation history on force and cytosolic CaPflugers Archiv : European journal of physiology · 2025Article
- Acute neuromuscular, cardiovascular, and muscle oxygenation responses to low-intensity aerobic interval exercises with blood flow restriction.Experimental physiology · 2024Article
- The addition of blood flow restriction during resistance exercise does not increase prolonged low-frequency force depression.Experimental physiology · 2024Article
- Determinants of the maximal functional reserve during repeated supramaximal exercise by humans: The roles of Nrf2/Keap1, antioxidant proteins, muscle phenotype and oxygenation.Redox biology · 2023Article
- Mitochondrial heterogeneity in diseases.Signal transduction and targeted therapy · 2023Review
- Natural Extracts Mitigate the Deleterious Effects of Prolonged Intense Physical Exercise on the Cardiovascular and Muscular Systems.Antioxidants (Basel, Switzerland) · 2023Article
- Eccentric exercise-induced muscle weakness amplifies the history dependence of force.European journal of applied physiology · 2023Article
- Endogenous and Exogenous Antioxidants in Skeletal Muscle Fatigue Development during Exercise.Antioxidants (Basel, Switzerland) · 2023Review
- Article
- Mechanisms of eccentric contraction-induced muscle damage and nutritional supplementations for mitigating it.Journal of muscle research and cell motility · 2022Review
- Neuromuscular Adaptations after an Altitude Training Camp in Elite Judo Athletes.International journal of environmental research and public health · 2021Article
- Fast skeletal muscle troponin activator CK-2066260 mitigates skeletal muscle weakness independently of the underlying cause.Journal of cachexia, sarcopenia and muscle · 2020Article
- Redox basis of exercise physiology.Redox biology · 2020Review
- Intramuscular mechanisms of overtraining.Redox biology · 2020Review
- Neutralizing mitochondrial ROS does not rescue muscle atrophy induced by hindlimb unloading in female mice.Journal of applied physiology (Bethesda, Md. : 1985) · 2020Article
- Alterations to neuromuscular properties of skeletal muscle are temporally dissociated from the oxygen uptake slow component.Scientific reports · 2020Article
3 more citing papers are in PubMed but not listed here.
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Skeletal muscle often shows a delayed force recovery after fatiguing stimulation, especially at low stimulation frequencies. In this study we focus on the role of reactive oxygen species (ROS) in this fatigue-induced prolonged low-frequency force depression. Intact, single muscle fibres were dissected from flexor digitorum brevis (FDB) muscles of rats and wild-type and superoxide dismutase 2 (SOD2) overexpressing mice. Force and myoplasmic free [Ca(2+)] ([Ca(2+)](i)) were measured. Fibres were stimulated at different frequencies before and 30 min after fatigue induced by repeated tetani. The results show a marked force decrease at low stimulation frequencies 30 min after fatiguing stimulation in all fibres. This decrease was associated with reduced tetanic [Ca(2+)](i) in wild-type mouse fibres, whereas rat fibres and mouse SOD2 overexpressing fibres instead displayed a decreased myofibrillar Ca(2+) sensitivity. The SOD activity was approximately 50% lower in wild-type mouse than in rat FDB muscles. Myoplasmic ROS increased during repeated tetanic stimulation in rat fibres but not in wild-type mouse fibres. The decreased Ca(2+) sensitivity in rat fibres could be partially reversed by application of the reducing agent dithiothreitol, whereas the decrease in tetanic [Ca(2+)](i) in wild-type mouse fibres was not affected by dithiothreitol or the antioxidant N-acetylcysteine. In conclusion, we describe two different causes of fatigue-induced prolonged low-frequency force depression, which correlate to differences in SOD activity and ROS metabolism. These findings may have clinical implications since ROS-mediated impairments in myofibrillar function can be counteracted by reductants and antioxidants, whereas changes in SR Ca(2+) handling appear more resistant to interventions.
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