ArticleClinical and experimental immunology2008
Honeybee venom secretory phospholipase A2 induces leukotriene production but not histamine release from human basophils.
Article in Clinical and experimental immunology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 43 citations in OpenAlex.
- The Responsiveness of Bee Venom Phospholipase A2 on Regulatory T Cells Correlates with the CD11cToxins · 2021Article
- Omics era in forensic medicine: towards a new ageTurkish journal of medical sciences · 2020Review
- Melittin fromAntibiotics (Basel, Switzerland) · 2020Article
- Secreted Phospholipase AJournal of immunology (Baltimore, Md. : 1950) · 2020Article
- Role of interleukin-18 in the pathophysiology of allergic diseases.Cytokine & growth factor reviews · 2016Review
- Review
- Review
- Anaphylaxis to insect venom allergens: role of molecular diagnostics.Current allergy and asthma reports · 2015Review
- Improved Therapeutic Profiles of PLA2-Free Bee Venom Prepared by Ultrafiltration Method.Toxicological research · 2015Article
- Mast cells and basophils are essential for allergies: mechanisms of allergic inflammation and a proposed procedure for diagnosis.Acta pharmacologica Sinica · 2013Review
- In vitro Biphasic Effect of Honey Bee Venom on Basophils from Screened Healthy Blood Donors.Allergy, asthma & immunology research · 2011Article
- Anti-inflammatory effect of Acacia visco extracts in animal models.Inflammopharmacology · 2010Article
- Expression of CD203c and CD63 in human basophils: relationship to differential regulation of piecemeal and anaphylactic degranulation processes.Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology · 2010Article
- Detection of honeybee venom in envenomed tissues by direct MALDI MSI.Journal of the American Society for Mass Spectrometry · 2009Article
- Allergy and the cardiovascular system.Clinical and experimental immunology · 2008Review
- Clinical immunology review series: an approach to the use of the immunology laboratory in the diagnosis of clinical allergy.Clinical and experimental immunology · 2008Review
- Clinical immunology review series: an approach to the patient with anaphylaxis.Clinical and experimental immunology · 2008Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The role of basophils in an anaphylactic response is well recognized but is usually masked by mast cells, which contain similar mediators for the induction of generalized vasodilatation and laryngeal constriction. The rapid onset of systemic anaphylactic symptoms, particularly in insect stings and ingested food, suggest that basophils, a circulating pool of cells containing histamine and other potent mediators such as leukotrienes, may be more involved in systemic anaphylaxis than originally thought. We wished to examine if secretory phospholipase A2, a systemic allergen found in honey bee venom (HBV-sPLA2) may activate basophils directly leading to rapid systemic mediator release. Basophils were isolated from human blood and stimulated with increasing concentrations of HBV-sPLA2. We found that physiological concentrations of HBV-sPLA2 induce rapid leukotriene C4 production from purified human basophils within 5 min, while interleukin (IL)-4 expression and production was induced at later time-points. Histamine release was not induced, signifying that HBV-sPLA2 did not induce generalized degranulation. Surface expression of CD63, CD69 and CD11b were up-regulated following HBV-sPLA2 treatment. Stimulation of basophils with anti-immunoglobulin E (IgE) following treatment with HBV-sPLA2 did not induce more leukotriene release. To investigate the mechanism of leukotriene production, 9-12 octadecadiynioc acid, a cyclooxygenase-1 (COX-1) and 15-lipoxygenase inhibitor, was used and this abrogated leukotriene production. These results indicate that HBV-sPLA2 can directly activate human basophils in vitro to induce leukotriene production.
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