ArticleEuropean biophysics journal : EBJ2008
Thickness distribution of actin bundles in vitro.
Article in European biophysics journal : EBJ, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 52 citations in OpenAlex.
- CaMKII generates actin bundle morphology and mechanics distinct from canonical bivalent cross-linkers.bioRxiv : the preprint server for biology · 2026Article
- Investigating active dynamics of contractile actomyosin gels with micro particle image velocimetry (micro-PIV) analysis.Scientific reports · 2026Article
- Visualizing Actin Packing and the Effects of Actin Attachment on Lipid Membrane Viscosity Using Molecular Rotors.JACS Au · 2024Article
- Actin Bundles Dynamics and Architecture.Biomolecules · 2023Review
- Hydrodynamic and Polyelectrolyte Properties of Actin Filaments: Theory and Experiments.Polymers · 2022Article
- Actin crosslinker competition and sorting drive emergent GUV size-dependent actin network architecture.Communications biology · 2021Article
- Regulation of Actin Bundle Mechanics and Structure by Intracellular Environmental Factors.Frontiers in physics · 2021Article
- Crowding tunes the organization and mechanics of actin bundles formed by crosslinking proteins.FEBS letters · 2021Article
- Semiflexible Biopolymers in Bundled Arrangements.Polymers · 2016Review
- The More the Tubular: Dynamic Bundling of Actin Filaments for Membrane Tube Formation.PLoS computational biology · 2016Article
- Physical model for the geometry of actin-based cellular protrusions.Biophysical journal · 2014Article
- Elasto-plastic response of reversibly crosslinked biopolymer bundles.The European physical journal. E, Soft matter · 2014Article
- Article
- Sarcomeric pattern formation by actin cluster coalescence.PLoS computational biology · 2012Article
- Modeling the formation of in vitro filopodia.Journal of mathematical biology · 2011Article
- Actin cross-linkers and the shape of stereocilia.Biophysical journal · 2010Article
- Cooperativity and frustration in protein-mediated parallel actin bundles.Physical review letters · 2009Article
- Traction forces during collective cell motion.HFSP journal · 2009Article
- Physical model for the width distribution of axons.The European physical journal. E, Soft matter · 2009Article
- Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bundles of filamentous actin form the primary building blocks of a broad range of cytoskeletal structures, including filopodia, stereocilia and microvilli. In each case, the cell uses specific associated proteins to tailor the dynamics, dimensions and mechanical properties of the bundles to suit a specific cellular function. While the length distribution of actin bundles was extensively studied, almost nothing is known about the thickness distribution. Here, we use high-resolution cryo-TEM to measure the thickness distribution of actin/fascin bundles, in vitro. We find that the thickness distribution has a prominent peak, with an exponential tail, supporting a scenario of an initial fast formation of a disc-like nucleus of short actin filaments, which only later elongates. The bundle thicknesses at steady state are found to follow the distribution of the initial nuclei indicating that no lateral coalescence occurs. Our results show that the distribution of bundles thicknesses can be controlled by monitoring the initial nucleation process. In vivo, this is done by using specific regulatory proteins complexes.
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