ArticleMolecular biology of the cell2008
Adducin promotes micrometer-scale organization of beta2-spectrin in lateral membranes of bronchial epithelial cells.
Article in Molecular biology of the cell, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 61 citations in OpenAlex.
- The membrane skeleton is constitutively remodeled in neurons by calcium signaling.Science (New York, N.Y.) · 2025Article
- Article
- Lack of adducin impairs the stability of endothelial adherens and tight junctions and may be required for cAMP-Rac1-mediated endothelial barrier stabilization.Scientific reports · 2022Article
- Variants in ADD1 cause intellectual disability, corpus callosum dysgenesis, and ventriculomegaly in humans.Genetics in medicine : official journal of the American College of Medical Genetics · 2022Article
- Loss of SPTBN1 Suppresses Autophagy Via SETD7-mediated YAP Methylation in Hepatocellular Carcinoma Initiation and Development.Cellular and molecular gastroenterology and hepatology · 2022Article
- βII spectrin (SPTBN1): biological function and clinical potential in cancer and other diseases.International journal of biological sciences · 2021Review
- The role of spectrin in cell adhesion and cell-cell contact.Experimental biology and medicine (Maywood, N.J.) · 2019Review
- Phosphorylation of adducin-1 by cyclin-dependent kinase 5 is important for epidermal growth factor-induced cell migration.Scientific reports · 2019Article
- Adducins inhibit lung cancer cell migration through mechanisms involving regulation of cell-matrix adhesion and cadherin-11 expression.Biochimica et biophysica acta. Molecular cell research · 2019Article
- Adducin-1 is essential for spindle pole integrity through its interaction with TPX2.EMBO reports · 2018Article
- Protein 4.1N is required for the formation of the lateral membrane domain in human bronchial epithelial cells.Biochimica et biophysica acta. Biomembranes · 2018Article
- A Review on Adducin from Functional to Pathological Mechanisms: Future Direction in Cancer.BioMed research international · 2018Review
- Adducin family proteins possess different nuclear export potentials.Journal of biomedical science · 2017Article
- NIMA-related kinase 1 (NEK1) regulates meiosis I spindle assembly by altering the balance between α-Adducin and Myosin X.PloS one · 2017Article
- Kv7 channels in the nucleus accumbens are altered by chronic drinking and are targets for reducing alcohol consumption.Addiction biology · 2016Article
- RNA sequencing analysis of human podocytes reveals glucocorticoid regulated gene networks targeting non-immune pathways.Scientific reports · 2016Article
- Feisty filaments: actin dynamics in the red blood cell membrane skeleton.Current opinion in hematology · 2016Review
- F-actin binding protein, anillin, regulates integrity of intercellular junctions in human epithelial cells.Cellular and molecular life sciences : CMLS · 2015Article
- Adducin is involved in endothelial barrier stabilization.PloS one · 2015Article
- Identification of distinct ChAT⁺ neurons and activity-dependent control of postnatal SVZ neurogenesis.Nature neuroscience · 2014Article
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
Adducin promotes assembly of spectrin-actin complexes, and is a target for regulation by calmodulin, protein kinase C, and rho kinase. We demonstrate here that adducin is required to stabilize preformed lateral membranes of human bronchial epithelial (HBE) cells through interaction with beta2-spectrin. We use a Tet-on regulated inducible small interfering RNA (siRNA) system to deplete alpha-adducin from confluent HBE cells. Depletion of alpha-adducin resulted in increased detergent solubility of spectrin after normal membrane biogenesis during mitosis. Conversely, depletion of beta2-spectrin resulted in loss of adducin from the lateral membrane. siRNA-resistant alpha-adducin prevented loss of lateral membrane, but only if alpha-adducin retained the MARCKS domain that mediates spectrin-actin interactions. Phospho-mimetic versions of adducin with S/D substitutions at protein kinase C phosphorylation sites in the MARCKS domain were not active in rescue. We find that adducin modulates long-range organization of the lateral membrane based on several criteria. First, the lateral membrane of adducin-depleted cells exhibited reduced height, increased curvature, and expansion into the basal surface. Moreover, E-cadherin-GFP, which normally is restricted in lateral mobility, rapidly diffuses over distances up to 10 microm. We conclude that adducin acting through spectrin provides a novel mechanism to regulate global properties of the lateral membrane of bronchial epithelial cells.
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