ArticlePloS one2007
Covalent attachment of proteins to solid supports and surfaces via Sortase-mediated ligation.
Article in PloS one, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
39 citing papers in PubMed, 113 citations in OpenAlex.
- Site-Specific Functionalization of Recombinant Spider Silk Using Enzymatic Sortase Coupling.ACS omega · 2025Article
- Biocatalytic Method for Producing an Affinity Resin for the Isolation of Immunoglobulins.Biomolecules · 2024Article
- Affinity Resins for the Isolation of Immunoglobulins G Obtained Using Biocatalytic Technology.International journal of molecular sciences · 2024Article
- SpSrtA-Catalyzed Isopeptide Ligation on Lysine Residues.Microorganisms · 2024Article
- Progress in affinity ligand-functionalized bacterial magnetosome nanoparticles for bio-immunomagnetic separation of HBsAg protein.PloS one · 2022Article
- New Paradigms of Pilus Assembly Mechanisms in Gram-Positive Actinobacteria.Trends in microbiology · 2020Review
- Enhancing Robustness of Sortase A by Loop Engineering and Backbone Cyclization.Chemistry (Weinheim an der Bergstrasse, Germany) · 2020Article
- Stable and selective permeable hydrogel microcapsules for high-throughput cell cultivation and enzymatic analysis.Microbial cell factories · 2020Article
- Biotin-Conjugated Cellulose Nanofibers Prepared via Copper-Catalyzed Alkyne-Azide Cycloaddition (CuAAC) "Click" Chemistry.Nanomaterials (Basel, Switzerland) · 2020Article
- Protein-Engineered Functional Materials.Advanced healthcare materials · 2019Review
- Broadening the scope of sortagging.RSC advances · 2019Review
- Improvement in catalytic activity and thermostability of a GH10 xylanase and its synergistic degradation of biomass with cellulase.Biotechnology for biofuels · 2019Article
- Biofabricating Functional Soft Matter Using Protein Engineering to Enable Enzymatic Assembly.Bioconjugate chemistry · 2018Review
- Linear array of multi-substrate tracts for simultaneous assessment of cell adhesion, migration, and differentiation.BioTechniques · 2017Article
- Site-Specific Protein Labeling via Sortase-Mediated Transpeptidation.Current protocols in protein science · 2017Article
- Peptide oligomers from ultra-short peptides using sortase.Biochemistry and biophysics reports · 2017Article
- Sortase A-mediated crosslinked short-chain dehydrogenases/reductases as novel biocatalysts with improved thermostability and catalytic efficiency.Scientific reports · 2017Article
- Proximity-Based Sortase-Mediated Ligation.Angewandte Chemie (International ed. in English) · 2017Article
- Article
- Efficient expression of sortase A fromBioresources and bioprocessing · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThere is growing interest in the attachment of proteins to solid supports for the development of supported catalysts, affinity matrices, and micro devices as well as for the development of planar and bead based protein arrays for multiplexed assays of protein concentration, interactions, and activity. A critical requirement for these applications is the generation of a stable linkage between the solid support and the immobilized, but still functional, protein. METHODOLOGY: Solid supports including crosslinked polymer beads, beaded agarose, and planar glass surfaces, were modified to present an oligoglycine motif to solution. A range of proteins were ligated to the various surfaces using the Sortase A enzyme of S. aureus. Reactions were carried out in aqueous buffer conditions at room temperature for times between one and twelve hours.
conclusionsThe Sortase A transpeptidase of S. aureus provides a general, robust, and gentle approach to the selective covalent immobilization of proteins on three very different solid supports. The proteins remain functional and accessible to solution. Sortase mediated ligation is therefore a straightforward methodology for the preparation of solid supported enzymes and bead based assays, as well as the modification of planar surfaces for microanalytical devices and protein arrays.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.