ArticleProceedings of the National Academy of Sciences of the United States of America2007
A distinct mechanism regulating a pollen-specific guanine nucleotide exchange factor for the small GTPase Rop in Arabidopsis thaliana.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 110 papers.
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Who cites it
110 citing papers in PubMed, 219 citations in OpenAlex.
- Identification of biogeographically informative microssatelite markers for Brazilian Cannabis sativa samples: a machine learning approach for forensic origin prediction.International journal of legal medicine · 2026Article
- Rab5 GTPases mediate the targeting of ROP signalling to establish polarity for pollen germination.Nature plants · 2025Article
- A GhSnRK10-GhRopGEF5-GhROP10 signaling pathway regulates cotton fiber cell growth.Plant communications · 2025Article
- RLCKs phosphorylate RopGEFs to control auxin-dependent Arabidopsis development.Nature plants · 2025Article
- OsPRK1/2/3-mediated reactive oxygen species signaling is required for pollen tube germination in rice.Journal of integrative plant biology · 2025Article
- Polarized subcellular activation of Rho proteins by specific ROPGEFs drives pollen germination in Arabidopsis thaliana.PLoS biology · 2025Article
- Polar localization and local translation of RHO-RELATED PROTEIN FROM PLANTS2 mRNAs promote root hair growth in Arabidopsis.The Plant cell · 2024Article
- Genome-wide association study of haploid female fertility (HFF) and haploid male fertility (HMF) in BS39-derived doubled haploid maize lines.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Review
- OsRopGEF10 Attenuates Cytokinin Signaling to Regulate Panicle Development and Grain Yield in Rice.Rice (New York, N.Y.) · 2024Article
- Arabidopsis class A S-acyl transferases modify the pollen receptors LIP1 and PRK1 to regulate pollen tube guidance.The Plant cell · 2024Article
- Article
- RHO OF PLANTS signalling and the activating ROP GUANINE NUCLEOTIDE EXCHANGE FACTORS: specificity in cellular signal transduction in plants.Journal of experimental botany · 2024Review
- TheInternational journal of molecular sciences · 2024Article
- Signaling network controlling ROP-mediated tip growth in Arabidopsis and beyond.Plant communications · 2023Review
- Let's shape again: the concerted molecular action that builds the pollen tube.Plant reproduction · 2022Review
- A small Rho GTPase OsRacB is required for pollen germination in rice.Development, growth & differentiation · 2022Article
- Interactome of Arabidopsis Thaliana.Plants (Basel, Switzerland) · 2022Article
- Calmodulin Domain Protein Kinase PiCDPK1 Regulates Pollen Tube Growth Polarity through Interaction with RhoGDI.Plants (Basel, Switzerland) · 2022Article
- Integrate QTL Mapping and Transcription Profiles Reveal Candidate Genes Regulating Flowering Time inFrontiers in plant science · 2022Article
- eIF4E1 RegulatesFrontiers in plant science · 2022Article
50 more citing papers are in PubMed but not listed here.
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rop/Rac small GTPases are central to diverse developmental and cellular activities in plants, playing an especially important role in polar growth of pollen tubes. Although it is established that a class of plant-specific RopGEFs promotes the activity of Rop/Rac through the catalytic PRONE (Plant-specific Rop nucleotide exchanger) domain, not much is known about how RopGEF function is controlled to allow a spatiotemporally regulated Rop activity. To understand such a process in pollen, we performed functional analysis with a pollen-specific RopGEF, AtRopGEF12. Overexpression of AtRopGEF12 had minimal phenotypic effects, whereas overexpression of a C-terminally truncated version disturbed tube growth, suggesting that the C terminus was inhibitory to GEF function. In contrast to non-pollen-expressed RopGEFs, pollen-expressed RopGEFs have conserved C termini. A phospho-mimicking mutation at an invariant serine within the C terminus of AtRopGEF12 resulted in loss of the C-terminal inhibition, suggesting that phosphorylation regulates GEF activity in vivo. The PRONE domain of AtRopGEF12 (PRONE12) was not sufficient to induce isotropic tube growth. We used mbSUS to show that AtRopGEF12 interacts with an Arabidopsis pollen receptor kinase AtPRK2a through its C terminus, and BiFC to show that they interact in pollen tubes. Coexpression of AtRopGEF12 and AtPRK2a caused isotropic growth reminiscent of that seen upon overexpression of a constitutively active (CA) Rop. Coexpression of AtPRK2a with an N-terminally truncated AtRopGEF12 did not induce isotropic growth, indicating a positive role for the N-terminal domain. Our results suggest a mechanism by which the noncatalytic domains of pollen-specific/enriched RopGEFs regulate PRONE function, leading to polarized pollen tube growth.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.